Journal article
In silico neuropeptidome of female Macrobrachium rosenbergii based on transcriptome and peptide mining of eyestalk, central nervous system and ovary
PLoS One, Vol.10(5), e0123848
2015
Abstract
Macrobrachium rosenbergii is the most economically important of the cultured freshwater crustacean species, yet there is currently a deficiency in genomic and transcriptomic information for research requirements. In this study, we present an in silico analysis of neuropeptide genes within the female M. rosenbergii eyestalk, central nervous system, and ovary. We could confidently predict 37 preproneuropeptide transcripts, including those that encode bursicons, crustacean cardioactive peptide, crustacean hyperglycemic hormones, eclosion hormone, pigment-dispersing hormones, diuretic hormones, neuropeptide F, neuroparsins, SIFamide, and sulfakinin. These transcripts are most prominent within the eyestalk and central nervous system. Transcript tissue distribution as determined by reverse transcription-polymerase chain reaction revealed the presence of selected neuropeptide genes of interest mainly in the nervous tissues while others were additionally present in the non-nervous tissues. Liquid chromatography-mass spectrometry analysis of eyestalk peptides confirmed the presence of the crustacean hyperglycemic hormone precursor. This data set provides a strong foundation for further studies into the functional roles of neuropeptides in M. rosenbergii, and will be especially helpful for developing methods to improve crustacean aquaculture.
Details
- Title
- In silico neuropeptidome of female Macrobrachium rosenbergii based on transcriptome and peptide mining of eyestalk, central nervous system and ovary
- Authors
- Saowaros Suwansa-ard (Author) - Mahidol University, ThailandT Thongbuakaew (Author) - Mahidol University, ThailandTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMin Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP J Hanna (Author) - Deakin UniversityP Sretarugsa (Author) - Mahidol University, ThailandScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP Sobhon (Author) - Mahidol University, Thailand
- Publication details
- PLoS One, Vol.10(5), e0123848; 31
- Publisher
- Public Library of Science
- Date published
- 2015
- DOI
- 10.1371/journal.pone.0123848
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2015 Suwansa-ard et al. This is an open access article distributed under the terms of theCreative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449953902621
- Output Type
- Journal article
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