In silico identification and in vivo analysis of a novel T-cell antigen from Chlamydia, NrdB

C J Barker; K W Beagley; L M Hafner; Peter Timms

doi:10.1016/j.vaccine.2007.12.048

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In silico identification and in vivo analysis of a novel T-cell antigen from Chlamydia, NrdB

Journal article

Peer reviewed

In silico identification and in vivo analysis of a novel T-cell antigen from Chlamydia, NrdB

C J Barker, K W Beagley, L M Hafner and Peter Timms

Vaccine, Vol.26(10), pp.1285-1296

2008

DOI: https://doi.org/10.1016/j.vaccine.2007.12.048

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Abstract

Chlamydia

vaccine

T-cell epitope

Chlamydial infections are a serious economic burden and health threat to developed and developing countries. Development of an efficacious vaccine is thought to be the most convenient, potentially reliable and cost effective option to control chlamydial infection and disease complications. Currently there are very few efficacious vaccine candidates that have been identified and characterized. In this study we have identified a number of unique vaccine candidates using a novel in silico approach. The chlamydial genome was screened for proteins containing epitopes predicted to bind multiple HLA class II molecules (i.e. 'promiscuous' epitopes). A selection of target proteins were cloned, expressed, and purified. Recombinant proteins were screened against sera samples from patients with Chlamydia trachomatis genital tract infections. Two proteins, hypothetical protein CT425 and ribonucleotide reductase small chain protein (NrdB) were identified as being immunoreactive. Using a mouse model, we found that intranasal immunization with NrdB conferred a CD4+ T-cell driven degree of protection similar to that seen with CD4+ T-cells primed from a whole organism, live challenge. In addition, serum from immunized mice was found to neutralize chlamydial infection of a cell monolayer in vitro. NrdB is a highly conserved chlamydial protein with an essential role in the replication of chlamydiae and could be a useful component of a multi-subunit vaccine against chlamydial genital tract infections. © 2008 Elsevier Ltd. All rights reserved.

Details

Title: In silico identification and in vivo analysis of a novel T-cell antigen from Chlamydia, NrdB
Authors: C J Barker (Author) - Queensland University of Technology
K W Beagley (Author) - Queensland University of Technology
L M Hafner (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: Vaccine, Vol.26(10), pp.1285-1296
Publisher: Elsevier Ltd.
Date published: 2008
DOI: 10.1016/j.vaccine.2007.12.048
ISSN: 0264-410X
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99448829002621
Output Type: Journal article

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