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Published VersionCC BY V4.0, Open Access
Journal article
In silico analysis of the slipper lobster (Thenus australiensis) neuropeptidome reveals unique enrichment of peptide hormones in reproductive tissues using multi-tissue transcriptomics
General and Comparative Endocrinology, Vol.375, pp.1-14
2026
PMID: 41271029
Abstract
The slipper lobster, Thenus australiensis has gained attention as a candidate species for aquaculture production. Recent studies have investigated various aspects of rearing T. australiensis in an aquaculture setting, however genetic resources are limited. Here, we generated the first multiple tissue transcriptome library for T. australiensis for immature male and female lobster tissues including the eyestalk, brain, testis, ovary, 3rd and 5th walking leg regions, hepatopancreas, stomach and muscle, with a total of 38 samples sequenced. From this transcriptome, we describe the neuropeptidome of T. australiensis and provide neuropeptide precursor expression patterns across tissues. The eyestalk and brain expressed the most neuropeptide precursor genes as expected, although surprisingly several others were enriched in the testis and ovary. Members of the allatostatin, crustacean hyperglycaemic hormone, and insulin like peptide family were among those highly expressed in gonad tissue, alongside peptides previously linked to reproduction such as corazonin and crustacean female sex hormone, and other neuropeptides without any established roles in reproduction. This repertoire of neuropeptides possibly related to reproductive processes provides a basis for future functional characterisation and may offer an avenue for enhancing production with aquaculture biotechnology.
Details
- Title
- In silico analysis of the slipper lobster (Thenus australiensis) neuropeptidome reveals unique enrichment of peptide hormones in reproductive tissues using multi-tissue transcriptomics
- Authors
- Thomas M. Banks - University of the Sunshine Coast, Queensland, Centre for BioinnovationSusan Glendinning - University of the Sunshine Coast, Queensland, Centre for BioinnovationCourtney Lewis - University of the Sunshine Coast, Queensland, Centre for BioinnovationAvani Bhojwani - University of the Sunshine Coast, Queensland, Centre for BioinnovationQuinn P. Fitzgibbon - University of TasmaniaGregory G. Smith - University of TasmaniaTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- General and Comparative Endocrinology, Vol.375, pp.1-14
- Publisher
- Academic Press
- Date published
- 2026
- DOI
- 10.1016/j.ygcen.2025.114855
- ISSN
- 1095-6840; 0016-6480
- PMID
- 41271029
- Copyright note
- © 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Data Availability
- Transcriptome data is available on the NCBI database (PRJNA772049), and on CrustyBase (https://crustybase.org/). Assembly and annotation files can be made available on request.
- Organisation Unit
- School of Education and Tertiary Access; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991184187402621
- Output Type
- Journal article
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