In Vitro primary human airway epithelial whole exhaust exposure

Katherine R Landwehr; Jessica Hillas; Ryan Mead-Hunter; Peter Brooks; Andrew King; Rebecca A O'Leary; Anthony Kicic; Benjamin J Mullins; Alexander N Larcombe

doi:10.1016/j.mex.2021.101561

Back

In Vitro primary human airway epithelial whole exhaust exposure

Journal article

Open access

Peer reviewed

In Vitro primary human airway epithelial whole exhaust exposure

Katherine R Landwehr, Jessica Hillas, Ryan Mead-Hunter, Peter Brooks, Andrew King, Rebecca A O'Leary, Anthony Kicic, Benjamin J Mullins and Alexander N Larcombe

MethodsX, Vol.8, pp.1-12

2021

DOI: https://doi.org/10.1016/j.mex.2021.101561

PMCID: PMC8563817

PMID: 34754823

Files and links (2)

pdf

In Vitro primary human airway epithelial whole exhaust exposure1.36 MBDownload View

Published VersionCC BY-NC-ND V4.0, Open Access

url

https://doi.org/10.1016/j.mex.2021.101561View

Published VersionCC BY-NC-ND V4.0, Open

Abstract

Airway epithelial cells

Biodiesel

Environmental exposure

Exhaust exposure

Exposure protocol

The method outlined in this article is a customization of the whole exhaust exposure method generated by Mullins et al. (2016) using reprogrammed primary human airway epithelial cells as described by Martinovich et al. (2017). It has been used successfully to generate recently published data (Landwehr et al. 2021). The goal was to generate an exhaust exposure model where exhaust is collected from a modern engine, real-world exhaust concentrations are used and relevant tissues exposed to assess the effects of multiple biodiesel exposures. Exhaust was generated, gently vacuumed into a dilution chamber where it was diluted 1/15 with air and then vacuumed into an incubator containing the primary cell cultures for exposure. Exhaust physico-chemical properties including combustion gas concentrations and particle spectra were then analyzed using a combustion gas analyzer and a Universal Scanning Mobility Particle Sizer. 24 h after exposure, cellular viability and mediator release were measured using Annexin-V/PI staining and meditator multiplexing kits respectively. This method was generated to test biodiesel exhaust exposures but can be easily adapted for any type of engine exhaust exposure or even potentially other respirable environmental exposures such as woodsmoke.

Details

Title: In Vitro primary human airway epithelial whole exhaust exposure
Authors: Katherine R Landwehr (Author) - Curtin University
Jessica Hillas (Author) - The Kids Research Institute Australia
Ryan Mead-Hunter (Author) - Curtin University
Peter Brooks (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Andrew King (Author) - Curtin University
Rebecca A O'Leary (Author) - Department of Primary Industries and Regional Development
Anthony Kicic (Author) - Curtin University
Benjamin J Mullins (Author) - Curtin University
Alexander N Larcombe (Author) - Curtin University
Contributors: AusREC (Research Group)
WAERP (Research Group)
Publication details: MethodsX, Vol.8, pp.1-12
Publisher: Elsevier BV
Date published: 2021
DOI: 10.1016/j.mex.2021.101561
ISSN: 2215-0161
PMID: 34754823; PMC8563817
Grants: A screening tool to identify safer biodiesels, DP170104346, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99584805302621
Output Type: Journal article

Metrics

2 File views/ downloads

20 Record Views

3 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Respiratory System

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites