In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain

B E Albrecht; U Breitenbach; T Stuhmer; Robert J Harvey; M G Darlison

doi:10.1111/j.1460-9568.1997.tb01658.x

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In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain

Journal article

Peer reviewed

In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain

B E Albrecht, U Breitenbach, T Stuhmer, Robert J Harvey and M G Darlison

European Journal of Neuroscience, Vol.9(11), pp.2414-2422

1997

DOI: https://doi.org/10.1111/j.1460-9568.1997.tb01658.x

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Abstract

Neurosciences

cerebellum

chicken (Gallus domesticus)

gene expression

optic tectum epithalamus

The pharmacological properties of homo-oligomeric channels formed by the GABA type A receptor-like pl and p2 polypeptides are very reminiscent of those of the GABA type C receptors that have been extensively characterized in the retina. Similar receptors have been reported to occur in certain brain regions of a variety of vertebrate species. We have used in situ hybridization to investigate the expression patterns of the zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBApl- and p2- polypeptide genes in the brain of the l-day-old chick (Gallus domesticus) and the adult rat (Ratfus norvegicus). Our results show that in the chick both the pl- and p2-subunit transcripts are present in the cerebellum, the optic tectum, the epithalamus and the nucleus pretectalis. However, the two messenger RNAs are often found in different populations of cells. Thus, only the pl-subunit gene is expressed in the deep cerebellar nuclei, the dorsal thalamus, the ectostriatum and the tractus vestibulomesencephalicus, while only the p2-subunit gene is transcribed in the nucleus habenularis lateralis and the nucleus isthmo-opticus. In contrast, neither of the p- polypeptide messenger RNAs can be detected by in sifu hybridization in the rat central nervous system. Reverse transcription-polymerase chain reaction amplification has been used to confirm the expression of the two p-subunit genes in the chicken brain. Surprisingly, this highly sensitive technique also revealed transcription of these genes in the rat brain. We conclude that the pl- and p2-subunit genes are expressed at a much higher level in the avian brain than in the rat brain and that, at least in birds, subtypes of the GAB& receptor exist.

Details

Title: In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain
Authors: B E Albrecht (Author) - lnstitut fur Herz- und Kreislaufforschung II, Germany
U Breitenbach (Author)
T Stuhmer (Author)
Robert J Harvey (Author) - Max-Planck-lnstitut fur Hirnforschun, Germany
M G Darlison (Author)
Publication details: European Journal of Neuroscience, Vol.9(11), pp.2414-2422
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 1997
DOI: 10.1111/j.1460-9568.1997.tb01658.x
ISSN: 0953-816X
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450459902621
Output Type: Journal article

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