In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes

Andrew Eamens; Neil A. Smith; Elizabeth S Dennis; Michael Wassenegger; Ming-Bo Wang

doi:10.1016/j.virol.2013.12.019

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In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes

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In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes

Andrew Eamens, Neil A. Smith, Elizabeth S Dennis, Michael Wassenegger and Ming-Bo Wang

Virology, Vol.450, pp.266-277

2014

DOI: https://doi.org/10.1016/j.virol.2013.12.019

PMID: 24503090

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Abstract

Potato spindle tuber viroid

Artificial microRNA

Small RNA

RNA silencing

Developmental phenotypes

Nicotiana

Soluble inorganic pyrophosphatase

Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s) resulting in symptom development. To test this, we expressed PSTVd sequences as artificial microRNAs (amiRNAs) in Nicotiana tabacum and Nicotiana benthamiana. One amiRNA, amiR46 that corresponds to sequences within the PSTVd virulence modulating region (VMR), induced abnormal phenotypes in both Nicotiana species that closely resemble those displayed by PSTVd infected plants. In N. tabacum amiR46 plants, phenotype severity correlated with amiR46 accumulation and expression down-regulation of the bioinformatically-identified target gene, a Nicotiana soluble inorganic pyrophosphatase (siPPase). Taken together, our phenotypic and molecular analyses suggest that disease symptom development in Nicotiana species following PSTVd infection results from sRNA-directed RNA silencing of the host gene, siPPase. (C) 2013 Elsevier Inc. All rights reserved.

Details

Title: In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes
Authors: Andrew Eamens (Author) - CSIRO Plant Industry
Neil A. Smith (Author) - CSIRO Plant Industry
Elizabeth S Dennis (Author) - CSIRO Plant Industry
Michael Wassenegger (Author) - Heidelberg University
Ming-Bo Wang (Author) - CSIRO Plant Industry
Publication details: Virology, Vol.450, pp.266-277
Publisher: Academic Press
DOI: 10.1016/j.virol.2013.12.019
ISSN: 1096-0341
PMID: 24503090
Organisation Unit: School of Health and Behavioural Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
Language: English
Record Identifier: 99656590702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Virology

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