Improving the case detection of pulmonary tuberculosis by bleach microscopy method in the North West of Nigeria

Ameh S James; Shuaibu Uba Abba; Aminu Ibrahm; Henry Mbah; Humphrey Musuluma; Kingsley Ochei; Saffiatou Darboe; Kwasi Torpey

doi:10.5897/JMLD2013.0066

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Improving the case detection of pulmonary tuberculosis by bleach microscopy method in the North West of Nigeria

Journal article

Open access

Improving the case detection of pulmonary tuberculosis by bleach microscopy method in the North West of Nigeria

Ameh S James, Shuaibu Uba Abba, Aminu Ibrahm, Henry Mbah, Humphrey Musuluma, Kingsley Ochei, Saffiatou Darboe and Kwasi Torpey

Journal of Medical Laboratory and Diagnosis, Vol.4(3), pp.34-37

2013

DOI: https://doi.org/10.5897/JMLD2013.0066

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Abstract

Medical Microbiology

Public Health and Health Services

bleach microscopy

tuberculosis

Nigeria

laboratory

Microscopy of direct smears for acid-fast bacilli (AFB) remains the common method for the laboratory diagnosis of pulmonary tuberculosis in most laboratories in our setting. The bleach microscopy method has been introduced and evaluated in different settings as an alternative to direct smear microscopy. However, this approach is yet to be evaluated in a typical government owned/public health laboratory in our setting thus the need for this study. This study was conducted in two Family Health International (FHI) supported health facilities in North West Nigeria. A total of 1075 sputum specimens were collected for the study, comprising of new and follow up cases, regardless of age group and sex. The sputa were examined by two methods, direct smear microscopy and household bleach and centrifugation method. Out of the 1075 sputum specimens, 171 specimens (40.3%) were positive for AFB by direct microscopy and 253 (59.7%) were positive for AFB by the household bleach method. There was a significant increase in the number of AFB positive specimens by the household bleach method (p less than 0.05). The bleach method significantly increase tuberculosis (TB) case detection compared to direct smear microscopy. The National TB and Leprosy Control Program could consider supporting the use of this method.

Details

Title: Improving the case detection of pulmonary tuberculosis by bleach microscopy method in the North West of Nigeria
Authors: Ameh S James (Author) - Family Health International, Nigeria
Shuaibu Uba Abba (Author) - Federal Medical Centre, Nigeria
Aminu Ibrahm (Author) - Sir Yaya Memorial Hospital, Nigeria
Henry Mbah (Author) - Family Health International, Nigeria
Humphrey Musuluma (Author) - Family Health International, Nigeria
Kingsley Ochei (Author) - Family Health International, Nigeria
Saffiatou Darboe (Author) - Medical Research Council Unit, Gambia
Kwasi Torpey (Author) - Family Health International, Nigeria
Publication details: Journal of Medical Laboratory and Diagnosis, Vol.4(3), pp.34-37
Publisher: Academic Journals
Date published: 2013
DOI: 10.5897/JMLD2013.0066
ISSN: 2141-2618
Copyright note: Copyright © 2013 Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99448745202621
Output Type: Journal article

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