Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells

Bianca M de Souza; Michelle Rodrigues; Fernanda S de Oliveira; Liana P A da Silva; A P Bouças; Ciro P Portinho; Bruno P dos Santos; Melissa Camassola; Dagoberto Rocha; Simone Lysakowski; Juliano Martini; Cristiane B Leitao; Nance B Nardi; Andrea C Bauer; Daisy Crispim

doi:10.1016/j.mce.2020.110729

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Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells

Journal article

Peer reviewed

Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells

Bianca M de Souza, Michelle Rodrigues, Fernanda S de Oliveira, Liana P A da Silva, A P Bouças, Ciro P Portinho, Bruno P dos Santos, Melissa Camassola, Dagoberto Rocha, Simone Lysakowski, …

Molecular and Cellular Endocrinology, Vol.505, 110729

2020

DOI: https://doi.org/10.1016/j.mce.2020.110729

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Abstract

Biological Sciences

Medical and Health Sciences

Agricultural and Veterinary Sciences

pancreatic islets

adipose-derived stem cells

co-culture

cytokines

gene expression

The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality.

Details

Title: Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells
Authors: Bianca M de Souza (Author) - Hospital de clinicas de Porto Alegre, Brazil
Michelle Rodrigues (Author) - Hospital de clinicas de Porto Alegre, Brazil
Fernanda S de Oliveira (Author) - Hospital de clinicas de Porto Alegre, Brazil
Liana P A da Silva (Author) - Hospital de clinicas de Porto Alegre, Brazil
A P Bouças (Author) - Universidade Federal do Rio Grande do Sul, Brazil
Ciro P Portinho (Author) - Hospital de clinicas de Porto Alegre, Brazil
Bruno P dos Santos (Author) - University of Bordeaux, France
Melissa Camassola (Author) - Universidade Luterana do Brasil, Brazil
Dagoberto Rocha (Author) - Universidade Federal de Ciências da Saúde de Porto Alegre, Brazil
Simone Lysakowski (Author) - Organ Procurement Organization, Brazil
Juliano Martini (Author) - Hospital Dom Vicente Scherer, Brazil
Cristiane B Leitao (Author) - Universidade Federal do Rio Grande do Sul, Brazil
Nance B Nardi (Author) - Universidade Luterana do Brasil, Brazil
Andrea C Bauer (Author) - Hospital de clinicas de Porto Alegre, Brazil
Daisy Crispim (Author) - Hospital de clinicas de Porto Alegre, Brazil
Publication details: Molecular and Cellular Endocrinology, Vol.505, 110729; 11
Publisher: Elsevier Ireland Ltd.
Date published: 2020
DOI: 10.1016/j.mce.2020.110729
ISSN: 0303-7207
Organisation Unit: University of the Sunshine Coast, Queensland; Thompson Institute
Language: English
Record Identifier: 99450820902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Cell Biology; Endocrinology & Metabolism

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