Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.

Hamish Cameron; Sylke H. Campion; Tripti Singh; Alankar A. Vaidya

doi:10.1007/s13205-014-0212-2

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Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.

Journal article

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Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.

Hamish Cameron, Sylke H. Campion, Tripti Singh and Alankar A. Vaidya

3 Biotech, Vol.5(2), pp.221-225

2015

DOI: https://doi.org/10.1007/s13205-014-0212-2

PMCID: PMC4362734

PMID: 28324577

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Abstract

accessory enzyme

fungi

hemicellulase

β-Mannanase

saccharification

softwood

Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, beta-mannanase. A Penicillium sp. was found to produce beta-mannanase in both solid (31.6 units/g of dry biomass) and liquid (117 units/g of dry biomass) cultures using locust bean gum as an inducer after 2 weeks of incubation. The saccharification of steam-exploded Pinus radiata was 7.8 % w/w improved when the crude extract of Penicillium sp. was added to a mixture of commercial enzymes.

Details

Title: Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
Authors: Hamish Cameron (Author) - University of Waikato
Sylke H. Campion (Author) - Scion
Tripti Singh (Author) - Scion
Alankar A. Vaidya (Corresponding Author) - Scion
Publication details: 3 Biotech, Vol.5(2), pp.221-225
Publisher: SpringerOpen
Date published: 2015
DOI: 10.1007/s13205-014-0212-2
ISSN: 2190-572X; 2190-5738
PMID: 28324577; PMC4362734
Copyright note: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Grant note: Scion core funding in Bioenergy
Organisation Unit: National Centre for Timber Durability and Design Life
Language: English
Record Identifier: 99979640102621
Output Type: Journal article

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