Impacts of Fsh, Igf1, and high temperature on the expression of genes involved in steroidogenesis, cell communication, and apoptosis in isolated coho salmon previtellogenic ovarian follicles

Kelli Anderson; Adam Luckenbach; Yoji Yamamoto; Abigail Elizur

doi:10.1016/j.aquaculture.2019.03.025

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Impacts of Fsh, Igf1, and high temperature on the expression of genes involved in steroidogenesis, cell communication, and apoptosis in isolated coho salmon previtellogenic ovarian follicles

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Impacts of Fsh, Igf1, and high temperature on the expression of genes involved in steroidogenesis, cell communication, and apoptosis in isolated coho salmon previtellogenic ovarian follicles

Kelli Anderson, Adam Luckenbach, Yoji Yamamoto and Abigail Elizur

Aquaculture, Vol.506, pp.60-69

2019

DOI: https://doi.org/10.1016/j.aquaculture.2019.03.025

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Abstract

In salmonids, exposure to elevatedtemperature impairsoogenesis. As such, there is a need to understand the molecular mechanisms that underpin this process, and develop mitigation strategies that maintain or rescue reproductive development inbroodstock.In thisstudy, follicle stimulating hormone (Fsh)and/or insulin-like growth factor1(Igf1) treatment were assessed for their ability to promotereproductive functionat 14 and 22 °C in ovarian follicles from coho salmon in vitro. Maintenance at 22°C resulted in the downregulation of fsh receptor, 17α-hydroxylase/C17,20-lyaseand p450 aromatase a(cyp19a1a), and connexin 34.3(cx34.3).While combined treatment with Fsh and Igf1stimulated the expression of cyp19a1aat 14 °C, thistreatment was not effective at 22 °C. Upregulation of cx34.3 occurred inresponse to treatments that contained Igf1regardless of temperature, and there is evidence to suggest that apoptosis was inhibited to some extent at 22 °C following combined treatment with Fsh and Igf1. This studydemonstratesthe thermal impairmentof key reproductive genes, and highlights the potential for novel hormone treatments to rescue oogenesis in salmonids.

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Title: Impacts of Fsh, Igf1, and high temperature on the expression of genes involved in steroidogenesis, cell communication, and apoptosis in isolated coho salmon previtellogenic ovarian follicles
Authors: Kelli Anderson (Author) - University of the Sunshine Coast - School of Science & Engineering
Adam Luckenbach (Author) - Washington State University, United States
Yoji Yamamoto (Author) - University of Washington, United States
Abigail Elizur (Author) - University of the Sunshine Coast - School of Science & Engineering
Publication details: Aquaculture, Vol.506, pp.60-69
Publisher: Elsevier BV
Date published: 2019
DOI: 10.1016/j.aquaculture.2019.03.025
ISSN: 0044-8486
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451119002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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