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Journal of Phycology - 2023 - Patwary - Identification of reproductive sex‐biased gene expression in Asparagopsis2.70 MB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Identification of reproductive sex‐biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes
Journal of Phycology, Vol.60(2), pp.327-342
2024
PMID: 38156746
Appears in UniSC Supported Open Access Outputs
Abstract
The sub‐tropical red seaweed Asparagopsis taxiformis is of significant interest due to its ability to store halogenated compounds, including bromoform, which can mitigate methane production in ruminants. Significant scale‐up of aquaculture production of this seaweed is required; however, relatively little is known about the molecular mechanisms that control fundamental physiological processes, including the regulatory factors that determine sexual dimorphism in gametophytes. In this study, we used comparative RNA‐sequencing analysis between different morphological parts of mature male and female A. taxiformis (lineage 6) gametophytes that resulted in greater number of sex‐biased gene expression in tips (containing the reproductive structures for both sexes), compared with the somatic main axis and rhizomes. Further comparative RNA‐seq against immature tips was used to identify 62 reproductive sex‐biased genes (59 male‐biased, 3 female‐biased). Of the reproductive male‐biased genes, 46% had an unknown function, while others were predicted to be regulatory factors and enzymes involved in signaling. We found that bromoform content obtained from female samples (8.5 ± 1.0 mg·g −1 dry weight) was ~10% higher on average than that of male samples (6.5 ± 1.0 mg·g −1 dry weight), although no significant difference was observed ( p > 0.05). There was also no significant difference in the marine bromoform biosynthesis locus gene expression. In summary, our comparative RNA‐sequencing analysis provides a first insight into the potential molecular factors relevant to gametogenesis and sexual differentiation in A. taxiformis , with potential benefits for identification of sex‐specific markers.
Details
- Title
- Identification of reproductive sex‐biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes
- Authors
- Zubaida Parveen Patwary (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringMin Zhao (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringNicholas A. Paul (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringScott F. Cummins (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Journal of Phycology, Vol.60(2), pp.327-342
- Publisher
- Wiley-Blackwell Publishing, Inc.
- Date published
- 2024
- DOI
- 10.1111/jpy.13419
- ISSN
- 1529-8817; 0022-3646
- PMID
- 38156746
- Copyright note
- This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2023 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America.
- Data Availability
- Raw data of all transcriptome sequencing was submitted to NCBI Bioproject and SRA database under a Bioproject accession number PRJNA869876 and PRJNA871928.
- Organisation Unit
- Cancer Research Cluster; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99987038402621
- Output Type
- Journal article
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