Identification of neuropeptides in sea cucumber Holothuria leucospilota

Chieu Hoang Dinh; Saowaros Suwansa-ard; Tianfang Wang; Abigail Elizur; Scott F Cummins

doi:10.1016/j.ygcen.2019.113229

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Identification of neuropeptides in sea cucumber Holothuria leucospilota

Journal article

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Identification of neuropeptides in sea cucumber Holothuria leucospilota

Chieu Hoang Dinh, Saowaros Suwansa-ard, Tianfang Wang, Abigail Elizur and Scott F Cummins

General and Comparative Endocrinology, Vol.283, 113229

2019

DOI: https://doi.org/10.1016/j.ygcen.2019.113229

PMID: 31348958

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Abstract

Neuropeptides play important roles in the regulation of physiological processes such as growth, metabolism and reproduction. In sea cucumbers (Phylum Echinodermata), numerous neuropeptides have been identified and some are attributed to reproductive processes. In this study, our goal was to gain a better understanding of the neuropeptide repertoire for the black sea cucumber Holothuria leucospilota, a species that has been severely overfished from the wild due to human consumption. We applied in silico transcriptome analysis of the adult H. leucospilota radial nerve cord, gonad and body wall to elucidate 37 neuropeptides that are conserved throughout the Bilateria. Then, liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of radial nerve cord was employed and showed an additional 8 putative novel neuropeptide precursors, whose predicative cleaved peptides do not share sequence similarity with any reported neuropeptides. These data provide an important basis for experimental approaches to manipulate H. leucospilota broodstock reproduction and growth in culture, which will hopefully re-establish population numbers.

Details

Title: Identification of neuropeptides in sea cucumber Holothuria leucospilota
Authors: Chieu Hoang Dinh (Author) - University of the Sunshine Coast
Saowaros Suwansa-ard (Author) - University of the Sunshine Coast
Tianfang Wang (Author) - University of the Sunshine Coast
Abigail Elizur (Author) - University of the Sunshine Coast
Scott F Cummins (Author) - University of the Sunshine Coast
Publication details: General and Comparative Endocrinology, Vol.283, 113229
Publisher: Academic Press
Date published: 2019
DOI: 10.1016/j.ygcen.2019.113229
ISSN: 0016-6480
PMID: 31348958
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451493602621
Output Type: Journal article

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Collaboration types: International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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