Identification of Genes Differentially Expressed in the Ganglia of Growing Haliotis asinina

P S York; Scott F Cummins; S M Degnan; B J Woodcroft; B M Degnan

doi:10.2983/035.029.0328

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Identification of Genes Differentially Expressed in the Ganglia of Growing Haliotis asinina

Journal article

Open access

Peer reviewed

Identification of Genes Differentially Expressed in the Ganglia of Growing Haliotis asinina

P S York, Scott F Cummins, S M Degnan, B J Woodcroft and B M Degnan

Journal of Shellfish Research, Vol.29(3), pp.741-752

2010

DOI: https://doi.org/10.2983/035.029.0328

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Abstract

abalone

growth

tropical abalone

Haliotis asinina

neuropeptide

differential expression

There is currently a paucity of specific genetic data regarding growth and reproduction-related processes in abalone, marine vetigastropods of commercial value. However, strong inferences about these processes can be drawn from other molluscs. For example, ganglia from the gastropods Aplysia and Lymnaea are known to produce neuropeptides related to growth, feeding behavior, and reproduction. Here, we use suppression subtractive hybridization to identify expressed genes that may be linked to the control of growth and feeding in the tropical abalone Haliotis asinina. Two pools of suppression subtractive hybridization clones were obtained from messenger RNA derived from H. asinina cerebral and pleuropedal ganglia, corresponding to genes differentially expressed in (1) well-nourished animals relative to food-deprived ones and (2) well-nourished animals relative to reproductively active ones. From these subtractions, respectively, 204 and 214 unigenes were identified in 222 and 231 sequenced clones, with 18 of these unigenes common to both subtractions. A subset of the putative differentially expressed genes was confirmed by quantitative polymerase chain reaction, validating this approach. The transcripts that are differentially expressed in the ganglia of growing H. asinina fall into a wide range of functional categories, including biological regulation, cell proliferation, and metabolic process, and include genes encoding Phe-Met-Arg-Phe-NH2 (or, FMRF-amide), myomodulin, CDC123, RAB37, and dermatopontin

Details

Title: Identification of Genes Differentially Expressed in the Ganglia of Growing Haliotis asinina
Authors: P S York (Author) - University of Queensland
Scott F Cummins (Author) - University of Queensland
S M Degnan (Author) - University of Queensland
B J Woodcroft (Author) - University of Queensland
B M Degnan (Author) - University of Queensland
Publication details: Journal of Shellfish Research, Vol.29(3), pp.741-752
Publisher: National Shellfisheries Association, Inc.
Date published: 2010
DOI: 10.2983/035.029.0328
ISSN: 0730-8000
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449457602621
Output Type: Journal article

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Web Of Science research areas: Fisheries; Marine & Freshwater Biology