Journal article
Identification of Genes Associated with Reproduction in the Mud Crab (Scylla olivacea) and Their Differential Expression following Serotonin Stimulation
PLoS One, Vol.9(12), e115867
2014
Abstract
The central nervous system (CNS) is often intimately involved in reproduction control and is therefore a target organ for transcriptomic investigations to identify reproduction-associated genes. In this study, 454 transcriptome sequencing was performed on pooled brain and ventral nerve cord of the female mud crab (Scylla olivacea) following serotonin injection (5 µg/g BW). A total of 197,468 sequence reads was obtained with an average length of 828 bp. Approximately 38.7% of 2,183 isotigs matched with significant similarity (E value < 1e-4) to sequences within the Genbank non-redundant (nr) database, with most significant matches being to crustacean and insect sequences. Approximately 32 putative neuropeptide genes were identified from nonmatching blast sequences. In addition, we identified full-length transcripts for crustacean reproductive-related genes, namely farnesoic acid o-methyltransferase (FAMeT), estrogen sulfotransferase (ESULT) and prostaglandin F synthase (PGFS). Following serotonin injection, which would normally initiate reproductive processes, we found up-regulation of FAMeT, ESULT and PGFS expression in the female CNS and ovary. Our data here provides an invaluable new resource for understanding the molecular role of the CNS on reproduction in S. olivacea.
Details
- Title
- Identification of Genes Associated with Reproduction in the Mud Crab (Scylla olivacea) and Their Differential Expression following Serotonin Stimulation
- Authors
- Napamanee Kornthong (Author) - Thammasat University, ThailandScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringCharoonroj Chotwiwatthanakun (Author) - Mahidol University, ThailandKanjana Khornchatri (Author) - Mahidol University, ThailandAttakorn Engsusophon (Author) - Mahidol University, ThailandPeter J Hanna (Author) - Mahidol University, ThailandPrasert Sobhon (Author) - Mahidol University, Thailand
- Publication details
- PLoS One, Vol.9(12), e115867; 21
- Publisher
- Public Library of Science
- Date published
- 2014
- DOI
- 10.1371/journal.pone.0115867
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2014 Kornthong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448649102621
- Output Type
- Journal article
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