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Identification of Gene Biomarkers for Tigilanol Tiglate Content in Fontainea picrosperma
Journal article   Open access   Peer reviewed

Identification of Gene Biomarkers for Tigilanol Tiglate Content in Fontainea picrosperma

Shahida A Mitu, Praphaporn Stewart, Trong Tran, Paul W Reddell, Scott Cummins and Steven Ogbourne
Molecules, Vol.27(13), pp.1-11
2022
DOI: https://doi.org/10.3390/molecules27133980
PMCID: PMC9268252
PMID: 35807225
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Identification of Gene Biomarkers for Tigilanol Tiglate Content in Fontainea picrosperma908.47 kBDownloadView
Published Version Open Access CC BY V4.0
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https://doi.org/10.3390/molecules27133980View
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Abstract

tigilanol tiglate anti-cancer biosynthesis pathway gene expression CYP94C1 R protein ERD-7.1 2-ARD stress drought
Tigilanol tiglate (EBC-46) is a small-molecule natural product under development for the treatment of cancers in humans and companion animals. The drug is currently produced by purification from the Australian rainforest tree Fontainea picrosperma (Euphorbiaceae). As part of a selective-breeding program to increase EBC-46 yield from F. picrosperma plantations, we investigated potential gene biomarkers associated with biosynthesis of EBC-46. Initially, we identified individual plants that were either high (>0.039%) or low EBC-46 (<0.008%) producers, then assessed their differentially expressed genes within the leaves and roots of these two groups by quantitative RNA sequencing. Compared to low EBC-46 producers, high-EBC-46-producing plants were found to have 145 upregulated genes and 101 downregulated genes in leaves and 53 upregulated genes and 82 downregulated genes in roots. Most of these genes were functionally associated with defence, transport, and biosynthesis. Genes identified as expressed exclusively in either the high or low EBC-46-producing plants were further validated by quantitative PCR, showing that cytochrome P450 94C1 in leaves and early response dehydration 7.1 and 2-alkenal reductase in roots were consistently and significantly upregulated in high-EBC-46 producers. In summary, this study has identified biomarker genes that may be used in the selective breeding of F. picrosperma.

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Biochemistry & Molecular Biology
Chemistry, Multidisciplinary

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