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Published VersionCC BY V4.0, Open Access
Journal article
Identification of Gene Biomarkers for Tigilanol Tiglate Content in Fontainea picrosperma
Molecules, Vol.27(13), pp.1-11
2022
Abstract
Tigilanol tiglate (EBC-46) is a small-molecule natural product under development for the treatment of cancers in humans and companion animals. The drug is currently produced by purification from the Australian rainforest tree Fontainea picrosperma (Euphorbiaceae). As part of a selective-breeding program to increase EBC-46 yield from F. picrosperma plantations, we investigated potential gene biomarkers associated with biosynthesis of EBC-46. Initially, we identified individual plants that were either high (>0.039%) or low EBC-46 (<0.008%) producers, then assessed their differentially expressed genes within the leaves and roots of these two groups by quantitative RNA sequencing. Compared to low EBC-46 producers, high-EBC-46-producing plants were found to have 145 upregulated genes and 101 downregulated genes in leaves and 53 upregulated genes and 82 downregulated genes in roots. Most of these genes were functionally associated with defence, transport, and biosynthesis. Genes identified as expressed exclusively in either the high or low EBC-46-producing plants were further validated by quantitative PCR, showing that cytochrome P450 94C1 in leaves and early response dehydration 7.1 and 2-alkenal reductase in roots were consistently and significantly upregulated in high-EBC-46 producers. In summary, this study has identified biomarker genes that may be used in the selective breeding of F. picrosperma.
Details
- Title
- Identification of Gene Biomarkers for Tigilanol Tiglate Content in Fontainea picrosperma
- Authors
- Shahida A Mitu (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyPraphaporn Stewart (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyTrong Tran (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyPaul W Reddell (Author) - EcoBiotics Ltd.Scott Cummins (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacySteven Ogbourne (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- Molecules, Vol.27(13), pp.1-11
- Publisher
- MDPI AG
- Date published
- 2022
- DOI
- 10.3390/molecules27133980
- ISSN
- 1420-3049
- Copyright note
- This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99654998602621
- Output Type
- Journal article
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