Journal article
Identification of Chlamydia pneumoniae proteins in the transition from reticulate to elementary body formation
Molecular and Cellular Proteomics, Vol.5(12), pp.2311-2318
2006
Abstract
Chlamydia pneumoniae is an important human respiratory pathogen that is responsible for an estimated 10% of community-acquired pneumonia and 5% of bronchitis and sinusitis cases. We examined changes in global protein expression profiles associated with the redifferentiation of reticulate body (RB) to elementary body (EB) as C. pneumoniae cells progressed from 24 to 48 h postinfection in HEp2 cells. Proteins corresponding to those showing the greatest changes in abundance in the beginning of the RB to EB transition were then identified from purified EBs. Among the 300 spots recognized, 35 proteins that were expressed at sufficiently high levels were identified by mass spectrometry. We identified C. pneumoniae proteins that showed more than 2-fold increases in abundance in the early stages of RB to EB transition, including several associated with amino acid and cofactor biosynthesis (Ndk, TrxA, Adk, PyrH, and BirA), maintenance of cytoplasmic protein function (GroEUES, DnaK, DksA, GrpE, HtrA, ClpP, ClpB, and Map), modification of the bacterial cell surface (CrpA, OmpA, and OmcB), energy metabolism (Tal and Pyk), and the putative transcriptional regulator TctD. This study identified C. pneumoniae proteins involved in the process of redifferentiation into mature, infective EBs and indicates bacterial metabolic pathways that may be involved in this transition. The proteins involved in RB to EB transition are key to C. pneumoniae infection and are perhaps suitable targets for therapeutic intervention. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
Details
- Title
- Identification of Chlamydia pneumoniae proteins in the transition from reticulate to elementary body formation
- Authors
- S Mukhopadhyay (Author) - University of Louisville, United StatesD Good (Author) - University of Louisville, United StatesR D Miller (Author) - University of Louisville, United StatesJ E Graham (Author) - University of Louisville, United StatesS A Mathews (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyJ T Summersgill (Author) - University of Louisville, United States
- Publication details
- Molecular and Cellular Proteomics, Vol.5(12), pp.2311-2318
- Publisher
- American Society for Biochemistry and Molecular Biology, Inc.
- Date published
- 2006
- DOI
- 10.1074/mcp.M600214-MCP200
- ISSN
- 1535-9476
- Copyright note
- Copyright © 2006 The American Society for Biochemistry and Molecular Biology. This research was originally published in Molecular and Cellular Proteomics. Mukhopadhyay, S, Good, D, Miller, R D, Graham, J E, Mathews, S A, Timms, Peter, Summersgill, J T. Identification of Chlamydia pneumoniae proteins in the transition from reticulate to elementary body formation. Molecular and Cellular Proteomics. 2006; Vol 5:2311-2318. © the American Society for Biochemistry and Molecular Biology.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449715102621
- Output Type
- Journal article
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