Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra

Napamanee Kornthong; Teva Phanaksri; Jirawat Saetan; Supawadee Duangprom; Buranee Lekskul; T Vivattanasarn; Sineenart Songkoomkrong; Prapaporn Jattujan; Scott Cummins; Prasert Sobhon; Saowaros Suwansa-ard

doi:10.1016/j.heliyon.2021.e08370

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Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra

Journal article

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Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra

Napamanee Kornthong, Teva Phanaksri, Jirawat Saetan, Supawadee Duangprom, Buranee Lekskul, T Vivattanasarn, Sineenart Songkoomkrong, Prapaporn Jattujan, Scott Cummins, Prasert Sobhon, …

Heliyon, Vol.7(11), pp.1-12

2021

DOI: https://doi.org/10.1016/j.heliyon.2021.e08370

PMCID: PMC8605306

PMID: 34825084

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Abstract

gene expression

growth factors

Holothuria scabra

sea cucumber

The sea cucumber Holothuria scabra is both an economically important species in Asian countries and an emerging experimental model for research studies in regeneration and medicinal bioactives. Growth factors and their receptors are known to be key components that guide tissue repair and renewal, yet validation of their presence in H. scabra has not been established. We performed a targeted in silico search of H. scabra transcriptome data to elucidate conserved growth factor family and receptor genes. In total, 42 transcripts were identified, of which 9 were validated by gene cloning and sequencing. The H. scabra growth factor genes, such as bone morphogenetic protein 2A (BMP 2A), bone morphogenetic protein 5-like (BMP5-like), neurotrophin (NT) and fibroblast growth factor 18 (FGF18), were selected for further analyses, including phylogenetic comparison and spatial gene expression using RT-PCR and in situ hybridization. Expression of all genes investigated were widespread in multiple tissues. However, BMP 2A, BMP5-like and NT were found extensively in the radial nerve cord cells, while FGF18 was highly expressed in connective tissue layer of the body wall. Our identification and expression analysis of the H. scabra growth factor genes provided the molecular information of growth factors in this species which may ultimately complement the research in regenerative medicine.

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Title: Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
Authors: Napamanee Kornthong (Author) - Thammasat University
Teva Phanaksri (Author) - Thammasat University Hospital
Jirawat Saetan (Author) - Prince of Songkla University
Supawadee Duangprom (Author) - Thammasat University
Buranee Lekskul (Author) - Thammasat University
T Vivattanasarn (Author) - Thammasat University
Sineenart Songkoomkrong (Author) - Thammasat University
Prapaporn Jattujan (Author) - Thammasat University
Scott Cummins (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Prasert Sobhon (Author) - Mahidol University
Saowaros Suwansa-ard (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Publication details: Heliyon, Vol.7(11), pp.1-12
Publisher: Elsevier Ltd
Date published: 2021
DOI: 10.1016/j.heliyon.2021.e08370
ISSN: 2405-8440
PMID: 34825084; PMC8605306
Copyright note: Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/)
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99586008102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology

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