Journal article
Identification and characterization of an insulin-like receptor involved in crustacean reproduction
Endocrinology, Vol.157(2), pp.928-941
2016
Abstract
Sexual differentiation and maintenance of masculinity in crustaceans has been suggested as being regulated by a single androgenic gland (AG) insulin-like peptide (IAG). However, downstream elements involved in the signaling cascade remain unknown. Here, we identified and characterized a gene encoding an insulin-like receptor in the prawn Macrobrachium rosenbergii (Mr-IR), the first such gene detected in a decapod crustacean. In mining for IRs and other insulin signaling related genes, we constructed a comprehensive M. rosenbergii transcriptomic library from multiple sources. In parallel we sequenced the complete Mr-IR cDNA, confirmed in the wide transcriptomic library. Mr-IR expression was detected in most tissues in both males and females, including the AG and gonads. To study Mr-IR function, we performed long-term RNAi silencing in young male prawns. While having no effect on growth, Mr-IR silencing advanced the appearance of a malespecific secondary trait. The most noted effects of Mr-IR silencing were hypertrophy of the AG and the associated increased production of Mr-IAG, with an unusual abundance of immature sperm cells being seen in the distal sperm duct. A ligand blot assay using de novo recombinant Mr-IAG confirmed the existence of a ligand-receptor interaction. While these results suggest a role for Mr-IR in the regulation of the AG, we did not see any sexual shift following silencing of Mr-IR, as occurred when the ligand-encoding Mr-IAG gene was silenced. This suggests that sexual differentiation in crustaceans involve more than a single Mr-IAG receptor, emphasizing the complexity of sexual differentiation and maintenance.
Details
- Title
- Identification and characterization of an insulin-like receptor involved in crustacean reproduction
- Authors
- O Sharabi (Author) - Ben-Gurion University of the Negev, IsraelR Manor (Author) - Ben-Gurion University of the Negev, IsraelS Weil (Author) - Ben-Gurion University of the Negev, IsraelE D Aflalo (Author) - Ben-Gurion University of the Negev, IsraelY Lezer (Author) - Ben-Gurion University of the Negev, IsraelT Levy (Author) - Ben-Gurion University of the Negev, IsraelJoseph Aizen (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP B Mather (Author) - Queensland University of TechnologyI Khalaila (Author) - Ben-Gurion University of the Negev, IsraelA Sagi (Author) - Ben-Gurion University of the Negev, Israel
- Publication details
- Endocrinology, Vol.157(2), pp.928-941
- Publisher
- Endocrine Society
- Date published
- 2016
- DOI
- 10.1210/en.2015-1391
- ISSN
- 0013-7227
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449184102621
- Output Type
- Journal article
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- Endocrinology & Metabolism
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