Identification and assessment of new vaccine candidates for group A streptococcal infections

David J McMillan; M R Batzloff; C L Browning; M R Davies; M F Good; K S Sriprakash; R Janulcsyk; M Rasmussen

doi:10.1016/j.vaccine.2004.01.043

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Identification and assessment of new vaccine candidates for group A streptococcal infections

Journal article

Peer reviewed

Identification and assessment of new vaccine candidates for group A streptococcal infections

David J McMillan, M R Batzloff, C L Browning, M R Davies, M F Good, K S Sriprakash, R Janulcsyk and M Rasmussen

Vaccine, Vol.22(21-22), pp.2783-2790

2004

DOI: https://doi.org/10.1016/j.vaccine.2004.01.043

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Abstract

Biological Sciences

Agricultural and Veterinary Sciences

Medical and Health Sciences

identification and assessment

new vaccine candidates

group A streptococcal infections

Group A Streptococcus (GAS) is a human-specific pathogen responsible for a wide variety of human diseases. Numerous GAS surface antigens interact with the human immune system and only some of these proteins have been studied in depth. A few of these may elicit protective response against GAS infection. In this study, we have used an in silico approach to identify antigenic peptides from GAS surface proteins. Putative GAS surface proteins from the M1 GAS genome were identified by the presence on LPxTG cell-wall anchoring motif and an export signal sequence. This technique identified 17 proteins of known or putative function, and another 11 which do not have known homologues. Peptides derived from predicted antigenic sequences near the amino terminus of six of these proteins, and another seven peptides derived from the two known surface proteins, GRAB and MtsA, were conjugated to keyhole lymphocyanin (KLH), and investigated for their capacity to induce opsonic antibody responses in outbred Quackenbush mice. All peptide-KLH antisera demonstrated opsonic capacity against both 88/30 and M1 GAS. However, KLH sera alone was also able to induce opsonic antibodies, suggesting that anti-KLH antibodies contributed to the opsonisation seen in the peptide-KLH antisera. KLH is therefore a promising carrier molecule for potential GAS peptide vaccines.

Details

Title: Identification and assessment of new vaccine candidates for group A streptococcal infections
Authors: David J McMillan (Author) - Queensland Institute of Medical Research
M R Batzloff (Author) - Queensland Institute of Medical Research
C L Browning (Author) - Queensland Institute of Medical Research
M R Davies (Author) - Queensland Institute of Medical Research
M F Good (Author) - Queensland Institute of Medical Research
K S Sriprakash (Author) - Queensland Institute of Medical Research
R Janulcsyk (Author) - Lund University, Sweden
M Rasmussen (Author) - Lund University, Sweden
Publication details: Vaccine, Vol.22(21-22), pp.2783-2790
Publisher: Elsevier Ltd.
Date published: 2004
DOI: 10.1016/j.vaccine.2004.01.043
ISSN: 0264-410X
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450298502621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology; Medicine, Research & Experimental

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