Journal article
Human and bovine adenoviruses for the detection of source-specific fecal pollution in coastal waters in Australia
Water Research, Vol.44(16), pp.4662-4673
2010
Abstract
In this study, the host-specificity and -sensitivity of human- and bovine-specific adenoviruses (HS-AVs and BS-AVs) were evaluated by testing wastewater/fecal samples from various animal species in Southeast, Queensland, Australia. The overall specificity and sensitivity of the HS-AVs marker were 1.0 and 0.78, respectively. These figures for the BS-AVs were 1.0 and 0.73, respectively. Twenty environmental water samples were collected during wet conditions and 20 samples were colleted during dry conditions from the Maroochy Coastal River and tested for the presence of fecal indicator bacteria (FIB), host-specific viral markers, zoonotic bacterial and protozoan pathogens using PCR/qPCR. The concentrations of FIB in water samples collected after wet conditions were generally higher compared to dry conditions. HS-AVs was detected in 20% water samples collected during wet conditions and whereas BS-AVs was detected in both wet (i.e., 10%) and dry (i.e., 10%) conditions. Both Campylobacter jejuni mapA and Salmonella invA genes detected in 10% samples collected during dry conditions. The concentrations of Salmonella invA ranged between 3.5 × 102 and 4.3 × 102 genomic copies per 500 ml of water Giardia lamblia β-giardin gene was detected only in one sample (5%) collected during the dry conditions. Weak or significant correlations were observed between FIB with viral markers and zoonotic pathogens. However, during dry conditions, no significant correlations were observed between FIB concentrations with viral markers and zoonotic pathogens. The prevalence of HS-AVs in samples collected from the study river suggests that the quality of water is affected by human fecal pollution and as well as bovine fecal pollution. The results suggest that HS-AVs and BS-AVs detection using PCR could be a useful tool for the identification of human sourced fecal pollution in coastal waters. © 2010.
Details
- Title
- Human and bovine adenoviruses for the detection of source-specific fecal pollution in coastal waters in Australia
- Authors
- Warish Ahmed (Author) - Queensland University of TechnologyA Goonetilleke (Author) - Queensland University of TechnologyTed Gardner (Author) - Queensland University of Technology
- Publication details
- Water Research, Vol.44(16), pp.4662-4673
- Publisher
- IWA Publishing
- Date published
- 2010
- DOI
- 10.1016/j.watres.2010.05.017
- ISSN
- 0043-1354
- Copyright note
- Copyright © 2010. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99451281002621
- Output Type
- Journal article
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