Human Chlamydia pneumoniae isolates demonstrate ability to recover infectivity following penicillin treatment whereas animal isolates do not

Anu Chacko; Kenneth W Beagley; Peter Timms; Wilhelmina M Huston

doi:10.1093/femsle/fnv015

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Human Chlamydia pneumoniae isolates demonstrate ability to recover infectivity following penicillin treatment whereas animal isolates do not

Journal article

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Human Chlamydia pneumoniae isolates demonstrate ability to recover infectivity following penicillin treatment whereas animal isolates do not

Anu Chacko, Kenneth W Beagley, Peter Timms and Wilhelmina M Huston

FEMS Microbiology Letters, Vol.362(6), pp.1-7

2015

DOI: https://doi.org/10.1093/femsle/fnv015

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Abstract

persistence

chlamydial

intracellular

stress response

Chlamydia pneumoniae strains have recently been demonstrated to have substantially different capacities to enter and recover from IFN-γ induced persistence, depending on whether they are from human or animal host sources. Here we examined the ability of two human and two animal strains to enter and be rescued from penicillin induced persistence. The ability to form inclusions after the addition of penicillin was much reduced in the two animal isolates (koala LPCoLN, bandicoot B21) compared to the two human isolates (respiratory AR39, heart A03). The penicillin treatment resulted in a dose dependent loss of infectious progeny for all isolates, with the human strains failing to produce infectious progeny at lower doses of penicillin than the animal strains. The most remarkable finding however was the contrasting ability of the isolates to recover infectious progeny production after rescue by removal of the penicillin (at 72 h) and continued culture. The animal isolates both showed virtually no recovery from the penicillin treatment conditions. In contrast, the human isolates showed a significant ability to recovery infectivity, with the heart isolate (A03) showing the most marked recovery. Combined, these data further support the hypothesis that the ability to establish and recover from persistence appears to be enhanced in human Chlamydia pneumoniae strains compared to animal strains.

Details

Title: Human Chlamydia pneumoniae isolates demonstrate ability to recover infectivity following penicillin treatment whereas animal isolates do not
Authors: Anu Chacko (Author) - Queensland University of Technology
Kenneth W Beagley (Author) - Queensland University of Technology
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wilhelmina M Huston (Author) - Queensland University of Technology
Publication details: FEMS Microbiology Letters, Vol.362(6), pp.1-7
Publisher: Oxford University Press
Date published: 2015
DOI: 10.1093/femsle/fnv015
ISSN: 0378-1097
Grants: Specificity of Chlamydia pneumoniae for humans: analysis of previous cross-host transmission events and the role of tryptophan availability in host specificity, 1004666, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Organisation Unit: Centre for Bioinnovation
Language: English
Record Identifier: 99449196202621
Output Type: Journal article

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