Host species-specific translocation of Escherichia coli

Mohammad Katouli; Nubia Ramos; C Nettelbladt; M Ljungdahl; Wayne A Robinson; Helen M Ison; A Brauner; R Mollby

doi:10.1007/s10096-009-0754-0

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Host species-specific translocation of Escherichia coli

Journal article

Peer reviewed

Host species-specific translocation of Escherichia coli

Mohammad Katouli, Nubia Ramos, C Nettelbladt, M Ljungdahl, Wayne A Robinson, Helen M Ison, A Brauner and R Mollby

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Vol.28(9), pp.1095-1103

2009

DOI: https://doi.org/10.1007/s10096-009-0754-0

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Abstract

translocation

Escherichia coli

The purpose of this paper is to investigate the rate of translocation of Escherichia coli strains in different experimental/animal models. Four proficient translocating E. coli strains isolated from mesenteric lymph nodes (MLNs) and/or the blood of rats (strains KIC-1 and KIC-2), from a fatal case of pancreatitis (HMLN-1) and from pigs (PC-1 isolated in this study) were tested for their ability to translocate across two host species and the Caco-2 cell line as a model of the human gut epithelium. HMLN-1 was found in the MLNs of all 15 pigs tested. This strain, however, did not translocate in any rats and only colonised the caecum of four rats in small numbers. HMLN-1 and PC-1 were the dominant translocating strains in Caco-2 cells compared to KIC-1 and KIC-2, which were found to translocate at a lower rate in pigs and in Caco-2 cells. The rate of translocation of PC-1 in rats was also very low compared to KIC-1 and KIC-2. We suggest that, in studies aiming to investigate the mechanism of translocation of E. coli strains isolated from humans, rats may not be an appropriate animal model and that the Caco-2 cells or pigs are more suitable in vitro and in vivo models, respectively.

Details

Title: Host species-specific translocation of Escherichia coli
Authors: Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Nubia Ramos (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
C Nettelbladt (Author) - Karolinska Hospital, Sweden
M Ljungdahl (Author) - University Hospital, Uppsala, Sweden
Wayne A Robinson (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Helen M Ison (Author) - University of the Sunshine Coast, Queensland
A Brauner (Author) - Karolinska Hospital, Sweden
R Mollby (Author) - Karolinska Hospital, Sweden
Publication details: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Vol.28(9), pp.1095-1103
Publisher: Springer
Date published: 2009
DOI: 10.1007/s10096-009-0754-0
ISSN: 0934-9723
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450111102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Infectious Diseases; Microbiology

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