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Host genetics, phenotype and geography structure the microbiome of a foundational seaweed
Journal article   Open access   Peer reviewed

Host genetics, phenotype and geography structure the microbiome of a foundational seaweed

G Wood, P D Steinberg, A H Campbell, A Vergés, M A Coleman and E M Marzinelli
Molecular Ecology, Vol.31(7), pp.2189-2206
2022
PMCID: PMC9540321
PMID: 35104026
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Host genetics, phenotype and geography structure the microbiome of a foundational seaweed1.90 MBDownloadView
Published Version Open Access CC BY V4.0
url
https://doi.org/10.1111/mec.16378View
Published Version

Abstract

Genetics phenotype macroalga kelp marine ecology Holobiont
Interactions between hosts and their microbiota are vital to the functioning and resilience of macro-organisms. Critically, for hosts that play foundational roles in communities, understanding what drives host-microbiota interactions is essential for informing ecosystem restoration and conservation. We investigated the relative influence of host traits and the surrounding environment on microbial communities associated with the foundational seaweed Phyllospora comosa. We quantified 16 morphological and functional phenotypic traits, including host genetics (using 354 Single Nucleotide Polymorphisms) and surface-associated microbial communities (using 16S rRNA-gene amplicon sequencing) from 160 individuals sampled from eight sites spanning Phyllospora's entire latitudinal distribution (1300 km). Combined, these factors explained 54% of the overall variation in Phyllospora's associated microbial community structure, much of which was related to the local environment (~32%). We found that putative "core" microbial taxa (i.e. present on all Phyllospora individuals sampled) exhibited slightly higher association with host traits when compared to "variable" taxa (not present on all individuals). We identified several key genetic loci and phenotypic traits in Phyllospora that were strongly related to multiple microbial amplicon sequence variants, including taxa with known associations to seaweed defense, disease and tissue degradation. This information on how host-associated microbial communities vary with host traits and the environment enhances our current understanding of how "holobionts" (hosts plus their microbiota) are structured. Such understanding can be used to inform management strategies of these important and vulnerable habitats.

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