Journal article
Histological studies of the ovaries of two tropical portunid crabs, Portunus pelagicus (L.) and Scylla serrata (F.)
Invertebrate Reproduction and Development, Vol.50(2), pp.85-97
2007
Abstract
The histology of ovaries of adult crabs, Portunus pelagicus and Scylla serrata, were studied and compared using light microscopy. In general, the histological features, the steps of oogenetic cells and the stages of ovarian development appear similar in the two species. The ovary possesses three to four lobes, and each lobe is divided into lobules. A thick capsule surrounds the ovarian lobes and its extensions cover and separate each lobule. The lobule is composed of follicles, each containing a cluster of oocytes at different steps within the confinement of connective tissue trabeculae. There are five steps of oogenetic cells, i.e., oogonia (Og) and four steps of oocytes (Oc
1
, Oc
2
, Oc
3
, Oc
4
). Og are the earliest germ cells and are located in the center of each lobule. They divide mitotically and move into each follicle where they become Oc
1
. Oc
2
rapidly increase in size, exhibits a clockface nucleus and basophilic cytoplasm. Oc
2
possess larger nuclei, non-condensed chromatin, a prominent nucleolus and are intensely basophilic. Oc
1
and Oc
2
are previtellogenic and do not contain yolk granules. Oc
3
are larger in size, with a slate grey cytoplasm and contain a few yolk granules. Oc
4
are fully mature oocytes, in possession of completely dispersed chromatin in the nucleus and a very prominent nucleolus. The cytoplasm is eosinophilic due to the presence of numerous yolk granules. The Periodic acid-Schiff reaction and Sudan black stains suggest that Oc
4
also contain large amounts of glycoprotein and lipid in their cytoplasm. The ovarian cycle is divided into four stages, i.e., spawn-spent (stage I), proliferative (stage II), pre-mature (stage III) and mature (stage IV). Each stage contains different cellular associations: stage I is characterized by the reconfiguration of connective tissue scaffold, plus remnants of unspawned oocytes; stage II contains numerous dividing Og and a few Oc
1
; stage III contains primarily Oc
1
, Oc
2
, Oc
3
; and stage IV contains mostly Oc
4
and small clusters of Og.
Details
- Title
- Histological studies of the ovaries of two tropical portunid crabs, Portunus pelagicus (L.) and Scylla serrata (F.)
- Authors
- Michael J Stewart (Author) - Deakin UniversityNantawan Soonklang (Author) - Thammasat UniversityPraphaporn Stewart (Author) - Deakin UniversityPeter J Hanna (Author) - School of Life and Environmental SciencesChaitip Wanichanon (Author) - Department of Anatomy, Faculty of Science , Mahidol UniversityAndrew Parratt (Author) - Deakin UniversityWei Duan (Author) - Deakin UniversityPrasert Sobhon (Corresponding Author) - Mahidol University
- Publication details
- Invertebrate Reproduction and Development, Vol.50(2), pp.85-97
- Publisher
- Taylor & Francis
- DOI
- 10.1080/07924259.2007.9652231
- ISSN
- 2157-0272
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99679198102621
- Output Type
- Journal article
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