Journal article
Highly Heterogeneous Probiotic Lactobacillus Species in Healthy Iranians with Low Functional Activities
PLoS One, Vol.10(12), e0144467
2015
Abstract
Background: Lactic acid bacteria (LAB) have been considered as potentially probiotic organisms due to their potential human health properties. This study aimed to evaluate both in vitro and in vivo, the potential probiotic properties of Lactobacillus species isolated from fecal samples of healthy humans in Iran. Methods and Results: A total of 470 LAB were initially isolated from 53 healthy individual and characterized to species level. Of these, 88 (86%) were Lactobacillus species. Biochemical and genetic fingerprinting with Phene-Plate system (PhP-LB) and RAPD-PCR showed that the isolates were highly diverse consisted of 67(76.1%) and 75 (85.2%) single types (STs) and a diversity indices of 0.994 and 0.997, respectively. These strains were tested for production of adhesion to Caco-2 cells, antibacterial activity, production of B12, anti-proliferative effect and interleukin-8 induction on gut epithelial cell lines and antibiotic resistance against 9 commonly used antibiotics. Strains showing the characteristics consistent with probiotic strains, were further tested for their anti-inflammatory effect in mouse colitis model. Only one L. brevis; one L. rhamnosus and two L. plantarum were shown to have significant probiotic properties. These strains showed shortening the length of colon compared to dextran sulfate sodium and disease activity index (DAI) was also significantly reduced in mouse. Conclusion: Low number of LAB with potential probiotic activity as well as high diversity of lactobacilli species was evident in Iranian population. It also suggest that specific strains of L. plantarum, L. brevis and L. rhamnosus with anti-inflammatory effect in mouse model of colitis could be used as a potential probiotic candidate in inflammatory bowel disease to decrease the disease activity index
Details
- Title
- Highly Heterogeneous Probiotic Lactobacillus Species in Healthy Iranians with Low Functional Activities
- Authors
- Mahdi Rohani (Author) - Pasteur Institute of Iran, IranNasrin Noohi (Author) - Pasteur Institute of Iran, IranMalihe Talebi (Author) - Iran University of Medical Sciences, IranMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMohammad R Pourshafie (Author) - Pasteur Institute of Iran, Iran
- Publication details
- PLoS One, Vol.10(12), e0144467; 18
- Publisher
- Public Library of Science
- Date published
- 2015
- DOI
- 10.1371/journal.pone.0144467
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2015 Rohani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449257802621
- Output Type
- Journal article
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