High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites

C Pasay; L Arlian; M Morgan; D Vyszenski-Moher; Annie Rose; D C Holt; Shelley F Walton; J McCarthy

doi:10.1111/j.1365-2915.2008.00716.x

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High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites

Journal article

Peer reviewed

High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites

C Pasay, L Arlian, M Morgan, D Vyszenski-Moher, Annie Rose, D C Holt, Shelley F Walton and J McCarthy

Medical and Veterinary Entomology, Vol.22(1), pp.82-88

2008

DOI: https://doi.org/10.1111/j.1365-2915.2008.00716.x

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Abstract

high-resolution melt analysis

knockdown resistance

permethrin

real-time PCR

scabies mite

voltage-gated sodium channel

Permethrin as a topical acaricide cream is widely used to treat scabies. The neuronal voltage-sensitive sodium channel (Vssc), necessary for the generation of action potentials in excitable cells, is the target of pyrethroid acaricides such as permethrin. Pyrethroid resistance has been linked to specific mutations in the Vssc gene. Following the partial sequencing of the Vssc gene in the scabies mite Sarcoptes scabiei (L.) (Astigmata: Sarcoptidae), we compared Vssc gene sequences from permethrin-sensitive and -tolerant S. scabiei var. canis Gerlach mites, and identified a G to A single nucleotide polymorphism (SNP) in permethrin-tolerant mites resulting in an amino acid change from glycine to aspartic acid in domain III S6. The mutation is in a region of the gene where mutations have been identified in a range of pyrethroid-resistant arthropods. Results of in vitro permethrin exposure assays showed that survival rates for mites bearing the mutation were similar to those previously reported for mites from human subjects where clinical tolerance to permethrin had been observed. A real-time polymerase chain reaction-high-resolution melt (PCR-HRM) assay was developed to detect this SNP. This assay provides a useful methodology for screening for this and other mutations associated with permethrin resistance in scabies mite populations and thus facilitates surveillance for acaricide resistance

Details

Title: High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites
Authors: C Pasay (Author) - University of Queensland
L Arlian (Author) - Wright State University, United States
M Morgan (Author) - Wright State University, United States
D Vyszenski-Moher (Author) - Wright State University, United States
Annie Rose (Author) - University of Queensland
D C Holt (Author) - Charles Darwin University
Shelley F Walton (Author) - Charles Darwin University
J McCarthy (Author) - University of Queensland
Publication details: Medical and Veterinary Entomology, Vol.22(1), pp.82-88
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2008
DOI: 10.1111/j.1365-2915.2008.00716.x
ISSN: 0269-283X
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
Language: English
Record Identifier: 99449426002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Entomology; Veterinary Sciences

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