Journal article
High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni
Applied and Environmental Microbiology, Vol.73(10), pp.3431-3436
2007
Abstract
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Details
- Title
- High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni
- Authors
- Erin P Price (Author) - Queensland University of TechnologyH Smith (Author) - Queensland Health Scientific ServicesF Huygens (Author) - Queensland University of TechnologyP M Giffard (Author) - Queensland University of Technology
- Publication details
- Applied and Environmental Microbiology, Vol.73(10), pp.3431-3436
- Publisher
- American Society for Microbiology
- Date published
- 2007
- DOI
- 10.1128/AEM.02702-06
- ISSN
- 0099-2240
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450563502621
- Output Type
- Journal article
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