Journal article
Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study
Journal of Nanoscience and Nanotechnology, Vol.5(6), pp.951-957
2005
Abstract
The atomic force microscopy has been used to analyze the immobilization of single stranded DNA on poly-L-lysine-coated glass and subsequent hybridization with complimentary DNA with the Z-threshold parameter and fractal analysis methods. The poly-L-lysine layer, which has a thickness of approximately 7 nm, presents nano-defects that could be critical for DNA immobilization by acting as a nucleation sites for ssDNA and subsequently for dsDNA aggregates. The Z-threshold for the dsDNA aggregates is much larger than for ssDNA, but the statistical fractal dimension is very similar, suggesting a conformal increase of the dimensions of the dsDNA aggregates mainly in the Z-direction, due to an effective ssDNA-ccDNA molecular recognition. This study demonstrates the use of fractal analysis in conjunction with the distribution of heights to evaluate the efficiency of DNA-DNA molecular recognition on surfaces and the impact of nanodefects. Copyright © 2005 American Scientific Publishers All rights reserved.
Details
- Title
- Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study
- Authors
- P D Sawant (Author)Gregory S Watson (Author)D Nicolau Jr (Author)S Myhra (Author)D V Nicolau (Author)
- Publication details
- Journal of Nanoscience and Nanotechnology, Vol.5(6), pp.951-957
- Publisher
- American Scientific Publishers
- Date published
- 2005
- DOI
- 10.1166/jnn.2005.125
- ISSN
- 1533-4880
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99449596002621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Chemistry, Multidisciplinary
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter
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Source: InCites