HPV16 E6 gene polymorphisms and the functions of the mutation site in cervical cancer among Uygur ethnic and Han nationality women in Xinjiang, China

Huizhen Xin; Zhenzhen Pan; Xiangyi Zhe; Chunhe Zhang; Hongtao Li; Weinan Zheng; Haichen Long; Renfu Shao; Dongmei Li; Zemin Pan

doi:10.1186/s12935-022-02506-0

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HPV16 E6 gene polymorphisms and the functions of the mutation site in cervical cancer among Uygur ethnic and Han nationality women in Xinjiang, China

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HPV16 E6 gene polymorphisms and the functions of the mutation site in cervical cancer among Uygur ethnic and Han nationality women in Xinjiang, China

Huizhen Xin, Zhenzhen Pan, Xiangyi Zhe, Chunhe Zhang, Hongtao Li, Weinan Zheng, Haichen Long, Renfu Shao, Dongmei Li and Zemin Pan

Cancer Cell International, Vol.22, pp.1-11

2022

DOI: https://doi.org/10.1186/s12935-022-02506-0

PMCID: PMC8862000

PMID: 35193568

Appears in UniSC Diversity and Inclusion Research Collection

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Abstract

HPV genotype

Sequencing analysis

HPV16 E6

Mutation

Cervical cancer

UniSC Diversity Area - Cultural and Linguistic Diversity

Background: To investigate the genotype distribution of human papillomavirus (HPV) in infected Uygur and Han women in Xinjiang, China; analyze the HPV16 E6 gene polymorphism site and relationship with the development of cervical cancer. Methods: The HPV16 E6 sequence was analyzed using the European standard prototype to perform an evolutionary tree. HPV16 E6-T295/T350, G295/G350, and T295/G350 GV230 vectors were stably transfected into cervical cancer C33A cells to analyze the cell proliferation, migration and invasion, apoptosis by CCK8 and clonogenic assays, tran-swell and cell scratch assays, FACS experiments. Results: The total HPV infection rate was 26.390% (760/2879), whereas the Uygur 22.87% (196/857) and the Han was 27.89% (564/2022) (P < 0.05). Among 110 mutations, 65 cases of E6 genes were mutated at nucleotide 350 (T350G) with the leucine changing to valine (L83V). Moreover, there were 7 cases of E6 gene mutated at nucleotide 295 (T295G) with aspartic changing to glutamic (D64E). When E6 vector(s) of mutations sites were transfected into C33A cells, they were found to promote cellular proliferation, migration, invasion, and inhibit apoptosis. T295/G350-E6 was significantly stronger than G295/G350 and T295/T350, G295/G350 was significantly stronger than T295/T350 (P < 0.05). The T295/G350 had the strongest effect on C33A cells and G295/G350 was significantly stronger than T295/T350 (P < 0.05). Conclusions: The positive HPV infection rates differed between the Uygur and Han in Xinjiang, China, and the genotype distribution of infection was different. After transfecting C33A cells with different eukaryotic expression vectors, the T295/G350 mutation site promoted the proliferation, migration, and invasion of C33A cells to a greater extent than G295/G350; however, G295/G350 had a stronger effect than T295/T350.

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Title: HPV16 E6 gene polymorphisms and the functions of the mutation site in cervical cancer among Uygur ethnic and Han nationality women in Xinjiang, China
Authors: Huizhen Xin (Author) - Shihezi University
Zhenzhen Pan (Author) - Xinjiang Production and Construction Corps
Xiangyi Zhe (Author) - Shihezi University
Chunhe Zhang (Author) - Shihezi University
Hongtao Li (Author) - Shihezi University
Weinan Zheng (Author) - Chengdu Medical College
Haichen Long (Author) - Shihezi University
Renfu Shao (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Dongmei Li (Author) - Shihezi University
Zemin Pan (Author) - Shihezi University
Publication details: Cancer Cell International, Vol.22, pp.1-11
Publisher: BioMed Central Ltd.
Date published: 2022
DOI: 10.1186/s12935-022-02506-0
ISSN: 1475-2867
PMID: 35193568; PMC8862000
Copyright note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99613308302621
Output Type: Journal article

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