HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples

Alyssa M Cornall; Marin Poljak; Suzanne M Garland; Samuel Phillips; Dorothy A Machalek; J. H. Tan; Michael A Quinn; Sepehr N Tabrizi

doi:10.1016/j.pvr.2017.10.002

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HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples

Journal article

Open access

Peer reviewed

HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples

Alyssa M Cornall, Marin Poljak, Suzanne M Garland, Samuel Phillips, Dorothy A Machalek, J. H. Tan, Michael A Quinn and Sepehr N Tabrizi

Papillomavirus Research, Vol.4, pp.79-84

2017

DOI: https://doi.org/10.1016/j.pvr.2017.10.002

PMID: 29179874

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Abstract

Anyplex II

Cervix

EuroArray

Genotyping

Human papillomavirus

Linear Array

Purpose To compare human papillomavirus genotype-specific performance of two genotyping assays, Anyplex II HPV28 (Seegene) and EuroArray HPV (EuroImmun), with Linear Array HPV (Roche). Methods DNA extracted from clinican-collected cervical brush specimens in PreservCyt medium (Hologic), from 403 women undergoing management for detected cytological abnormalities, was tested on the three assays. Genotype-specific agreement were assessed by Cohen's kappa statistic and Fisher's z-test of significance between proportions. Results Agreement between Linear Array and the other 2 assays was substantial to almost perfect (κ = 0.60 − 1.00) for most genotypes, and was almost perfect (κ = 0.81 – 0.98) for almost all high-risk genotypes. Linear Array overall detected most genotypes more frequently, however this was only statistically significant for HPV51 (EuroArray; p = 0.0497), HPV52 (Anyplex II; p = 0.039) and HPV61 (Anyplex II; p=0.047). EuroArray detected signficantly more HPV26 (p = 0.002) and Anyplex II detected more HPV42 (p = 0.035) than Linear Array. Each assay performed differently for HPV68 detection: EuroArray and LA were in moderate to substantial agreement with Anyplex II (κ = 0.46 and 0.62, respectively), but were in poor disagreement with each other (κ = −0.01). Conclusions EuroArray and Anyplex II had similar sensitivity to Linear Array for most high-risk genotypes, with slightly lower sensitivity for HPV 51 or 52.

Details

Title: HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples
Authors: Alyssa M Cornall (Corresponding Author) - The University of Melbourne
Marin Poljak - Murdoch Children's Research Institute
Suzanne M Garland - Royal Women's Hospital
Samuel Phillips - Royal Women's Hospital
Dorothy A Machalek - Murdoch Children's Research Institute
J. H. Tan - The University of Melbourne
Michael A Quinn - Royal Women's Hospital
Sepehr N Tabrizi - The University of Melbourne
Publication details: Papillomavirus Research, Vol.4, pp.79-84
Publisher: Elsevier BV
Date published: 2017
DOI: 10.1016/j.pvr.2017.10.002
ISSN: 2405-8521
PMID: 29179874
Copyright note: © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/)
Organisation Unit: School of Health - Biomedicine; Centre for Bioinnovation
Language: English
Record Identifier: 991144232402621
Output Type: Journal article

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