Gonadotropins β-GtHI and β-GtHII from the Gilthead Seabream, Sparus aurata

Abigail Elizur; N Zmora; H Rosenfeld; I Meiri; S Hassin; H Gordin; Y Zohar

doi:10.1006/gcen.1996.0044

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Gonadotropins β-GtHI and β-GtHII from the Gilthead Seabream, Sparus aurata

Journal article

Peer reviewed

Gonadotropins β-GtHI and β-GtHII from the Gilthead Seabream, Sparus aurata

Abigail Elizur, N Zmora, H Rosenfeld, I Meiri, S Hassin, H Gordin and Y Zohar

General and Comparative Endocrinology, Vol.102(1), pp.39-46

1996

DOI: https://doi.org/10.1006/gcen.1996.0044

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Abstract

Clinical Sciences

gonadotropins

reproduction

seabream

Sparus aurata

Full-length cDNA sequences encoding the β-subunits of the gonadotropins GtHI and GtHII were isolated, cloned, and sequenced from a single gilthead seabream (Sparus aurata) pituitary using RACE PCR. β-GtHI and β-GtHII degenerate PCR primers were designed according to regions of high amino acid sequence homology between the chum salmon and the bonito β-GtHI or β-GtHII. DNA sequence analysis of the cloned PCR products confirmed the presence of the predicted complete coding region as well as 5â€² and 3â€² untranslated sequences. The deduced amino acid sequences of β-GtHI and β-GtHII from the gilthead seabream were compared to GtH sequences from a number of teleosts. Seabream β-GtHI shows the highest homology to the bonito β-GtHI (76%) and the striped bass (74%), while seabream β-GtHII is 98% homologous to the yellow fin porgy, 87% homologous to the bonito, and 93% homologous to the striped bass β-GtHII. Northern blot analysis showed the transcripts of the gilthead seabream β-GtHI and β-GtHII to be about 700 and 740 bases, respectively. During the spawning season, β-GtHI is expressed at higher levels in males than in females while β-GtHII is expressed at similar levels in both sexes.

Details

Title: Gonadotropins β-GtHI and β-GtHII from the Gilthead Seabream, Sparus aurata
Authors: Abigail Elizur (Author) - Israel Oceanographic and Limnological Research, Israel
N Zmora (Author) - Israel Oceanographic and Limnological Research, Israel
H Rosenfeld (Author) - Israel Oceanographic and Limnological Research, Israel
I Meiri (Author) - Israel Oceanographic and Limnological Research, Israel
S Hassin (Author) - University of Maryland, United States
H Gordin (Author) - Israel Oceanographic and Limnological Research, Israel
Y Zohar (Author) - University of Maryland, United States
Publication details: General and Comparative Endocrinology, Vol.102(1), pp.39-46
Publisher: Academic Press
Date published: 1996
DOI: 10.1006/gcen.1996.0044
ISSN: 0016-6480
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449257002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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