Journal article
Gonadotropin-releasing hormone and adipokinetic hormone/corazonin-related peptide in the female prawn
General and Comparative Endocrinology, Vol.236, pp.70-82
2016
Abstract
Crustacean neuropeptides (NPs) play important roles in the regulation of most physiological activities, including growth, molting and reproduction. In this study, we have performed an in silico analysis of female prawn (Macrobrachium rosenbergii) neural transcriptomes to identify NPs not previously identified. We predict that approximately 1,309 proteins are destined for the secretory pathway, many of which are likely post-translationally processed to generate active peptides. Within this neural secretome, we identified a gene transcript that encoded a precursor protein with striking similarity to a gonadotropin-releasing hormone (GnRH). We additionally identified another GnRH NP superfamily member, the adipokinetic hormone/corazonin-related peptide (ACP). M. rosenbergii GnRH and ACP were widespread throughout the nervous tissues, implicating them as potential neuromodulators. Furthermore, GnRH was found in non-neural tissues, including the stomach, gut, heart, testis and ovary, in the latter most prominently within secondary oocytes. The GnRH/corazonin receptor-like gene is specific to the ovary, whereas ACP receptor-like gene expression is more widespread. Administration of GnRH had no effect on ovarian development and maturation, nor any effect on total hemolymph lipid levels, while ACP administration decreased oocyte proliferation (at high dose) and stimulated significant increase in total hemolymph lipids. In conclusion, our targeted analysis of the M. rosenbergii neural secretome has revealed the decapod GnRH and ACP genes. We propose that ACP in crustaceans plays a role in the lipid metabolism and the inhibition of oocyte proliferation, while the role of the GnRH remains to be clearly defined, possibly through experiments involving gene silencing.
Details
- Title
- Gonadotropin-releasing hormone and adipokinetic hormone/corazonin-related peptide in the female prawn
- Authors
- Saowaros Suwansa-ard (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMin Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTipsuda Thongbuakaew (Author) - Mahidol University, ThailandPiyachat Chansela (Author) - Phramongkutklao College of Medicine, ThailandTomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringPrasert Sobhon (Author) - Burapha University, Thailand
- Publication details
- General and Comparative Endocrinology, Vol.236, pp.70-82
- Publisher
- Academic Press
- Date published
- 2016
- DOI
- 10.1016/j.ygcen.2016.07.008
- ISSN
- 0016-6480
- Copyright note
- Copyright © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450446602621
- Output Type
- Journal article
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- Domestic collaboration
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- Web Of Science research areas
- Endocrinology & Metabolism
- Zoology
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