Journal article
Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration
Biology of Reproduction, Vol.100(3), pp.798-809
2019
Abstract
The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in yeast. Its receptor binding capacity and steroidogenic potency were confirmed in vitro. Weekly injections of rggFSH to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, BW) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs. primary growth stage in control). Sustained treatment with rggFSH (20 to 38 weeks at 200 μg/kg BW) resulted in significant reduction in gonad size, degeneration of oocytes and proliferation of spermatogonial cells, indicative of female to male sex change. Gene expression analysis showed that, while initiating female to male sex change, the rggFSH significantly suppressed the steroidogenic genes cyp11b, cyp19a1a and foxl2 which restrained the endogenous production of sex steroid hormones thus prevented the differentiation of spermatogonial cells. Expression profile of sex markers dmrt1, amh, figla and bmp15 suggests that the observed sex change was restricted at the initiation stage. Based on these results, we propose that the process of female to male sex change in the protogynous grouper is initiated by FSH, rather than sex steroids and likely involves steroid-independent pathway. The cortical alveolar stage in oocyte development is the critical point after which FSHinduced sex change is possible in grouper.
Details
- Title
- Gonadal response of juvenile protogynous grouper (Epinephelus fuscoguttatus) to long term recombinant follicle-stimulating hormone administration
- Authors
- Peter Palma (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyJosephine N Nocillado (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyJoshua Superio (Author) - Southeast Asian Fisheries Development Center, PhilippinesEvelyn Grace de Jesus Ayson (Author) - Southeast Asian Fisheries Development Center, PhilippinesFelix Ayson (Author) - Southeast Asian Fisheries Development Center, PhilippinesIdo Bar (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyAbigail Elizur (Corresponding Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- Biology of Reproduction, Vol.100(3), pp.798-809
- Publisher
- Oxford University Press
- Date published
- 2019
- DOI
- 10.1093/biolre/ioy228
- ISSN
- 0006-3363
- Copyright note
- Copyright © 2019 Oxford University Press. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Biology of Reproduction following peer review. The definitive publisher-authenticated version is available online at: http://dx.doi.org/10.1093/biolre/ioy228
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450655702621
- Output Type
- Journal article
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