Glycine receptors containing the α4 subunit in the embryonic sympathetic nervous system, spinal cord and male genital ridge

Robert J Harvey; V Schmieden; A Von Holst; B Laube; H Rohrer; H Betz

doi:10.1046/j.1460-9568.2000.00993.x

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Glycine receptors containing the α4 subunit in the embryonic sympathetic nervous system, spinal cord and male genital ridge

Journal article

Peer reviewed

Glycine receptors containing the α4 subunit in the embryonic sympathetic nervous system, spinal cord and male genital ridge

Robert J Harvey, V Schmieden, A Von Holst, B Laube, H Rohrer and H Betz

European Journal of Neuroscience, Vol.12(3), pp.994-1001

2000

DOI: https://doi.org/10.1046/j.1460-9568.2000.00993.x

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Abstract

dorsal root ganglion

excitatory glycine receptor

glial cells

in situ hybridization

sex cords

Inhibitory glycine receptors (GlyRs) are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions. Several developmentally and regionally regulated GlyR isoforms exist, which result from a differential expression of the GlyR α (α1-α4) and β subunit genes. Currently, very little is known about GlyRs containing the α4 subunit, whose existence was predicted from a partial genomic sequence. Here, we describe the isolation of complementary DNA (cDNA) sequences for the mouse and chick GlyR α4 subunits. We show that a mouse GlyR α4 subunit full-length cDNA directs the formation of functional homo-oligomeric strychnine-sensitive GlyRs in Xenopus laevis oocytes and mammalian cells, and that these resemble GlyRs composed of the α1 subunit in pharmacological profile and single-channel properties. In situ hybridization reveals high levels of GlyR α4 subunit transcripts in the embryonic (E13) chick spinal cord, lumbosacral sympathetic ganglia and dorsal root ganglia. The avian GlyR α4 subunit gene also shows male-specific expression in the developing genital ridge. The pharmacological profile of α4 subunit-containing receptors and deduced location of the avian GlyR α4 subunit are consistent with it being a component of the embryonic excitatory GlyRs previously identified in sympathetic neurons. Our data also suggest a novel role for GlyRs in the maturation of reproductive organs.

Details

Title: Glycine receptors containing the α4 subunit in the embryonic sympathetic nervous system, spinal cord and male genital ridge
Authors: Robert J Harvey (Author) - Max-Planck-Institut fuÈr Hirnforschung, Germany
V Schmieden (Author) - UniversitaÈts-Klinikum Charite, Germany
A Von Holst (Author) - Max-Planck-Institut fuÈr Hirnforschung, Germany
B Laube (Author) - Max-Planck-Institut fuÈr Hirnforschung, Germany
H Rohrer (Author) - Max-Planck-Institut fuÈr Hirnforschung, Germany
H Betz (Author) - Max-Planck-Institut fuÈr Hirnforschung, Germany
Publication details: European Journal of Neuroscience, Vol.12(3), pp.994-1001
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2000
DOI: 10.1046/j.1460-9568.2000.00993.x
ISSN: 0953-816X
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450437002621
Output Type: Journal article

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