Journal article
Genotypic variability enhances the reproducibility of an ecological study
Nature Ecology and Evolution, Vol.2(2), pp.279-287
2018
Abstract
Many scientific disciplines are currently experiencing a 'reproducibility crisis' because numerous scientific findings cannot be repeated consistently. A novel but controversial hypothesis postulates that stringent levels of environmental and biotic standardization in experimental studies reduce reproducibility by amplifying the impacts of laboratory-specific environmental factors not accounted for in study designs. A corollary to this hypothesis is that a deliberate introduction of controlled systematic variability (CSV) in experimental designs may lead to increased reproducibility. To test this hypothesis, we had 14 European laboratories run a simple microcosm experiment using grass (Brachypodium distachyon L.) monocultures and grass and legume (Medicago truncatula Gaertn.) mixtures. Each laboratory introduced environmental and genotypic CSV within and among replicated microcosms established in either growth chambers (with stringent control of environmental conditions) or glasshouses (with more variable environmental conditions). The introduction of genotypic CSV led to 18% lower among-laboratory variability in growth chambers, indicating increased reproducibility, but had no significant effect in glasshouses where reproducibility was generally lower. Environmental CSV had little effect on reproducibility. Although there are multiple causes for the 'reproducibility crisis', deliberately including genetic variability may be a simple solution for increasing the reproducibility of ecological studies performed under stringently controlled environmental conditions. © 2018 The Author(s) 2017, under exclusive licence to Macmillan Publishers Limited, part of Springer Nature.
Details
- Title
- Genotypic variability enhances the reproducibility of an ecological study
- Authors
- A Milcu (Author)R Puga-Freitas (Author)Aaron M Ellison (Author) - University of the Sunshine Coast - Faculty of Arts, Business and LawM Blouin (Author)S Scheu (Author)G T Freschet (Author)L Rose (Author)S Barot (Author)S Cesarz (Author)N Eisenhauer (Author)T Girin (Author)D Assandri (Author)M Bonkowski (Author)N Buchmann (Author)O Butenschoen (Author)S Devidal (Author)G Gleixner (Author)Carlo Grignani (Author)A Gessler (Author)Amandine Hansart (Author)Zachary Kayler (Author)A Gigon (Author)A Greiner (Author)Markus Lange (Author)Jean-Christophe Lata (Author)Jean-Francois Le Galliard (Author)Martin Lukac (Author)Neringa Mannerheim (Author)Marina E H Muller (Author)Anne Pando (Author)Paula Rotter (Author)Michael Scherer-Lorenzen (Author)Rahme Seyhun (Author)Katherine Urban-Mead (Author)Alexandra Weigelt (Author)Laura Zavattaro (Author)Jacques Roy (Author)
- Publication details
- Nature Ecology and Evolution, Vol.2(2), pp.279-287
- Publisher
- Nature Publishing Group
- Date published
- 2018
- DOI
- 10.1038/s41559-017-0434-x
- ISSN
- 2397-334X
- Copyright note
- Copyright © 2018 Nature Publishing Group. The accepted version is reproduced here in accordance with the publishers copyright policy. The definitive version is available from https://www.nature.com/natecolevol/
- Organisation Unit
- Tropical Forests and People Research Centre; University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99451495002621
- Output Type
- Journal article
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