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Published VersionCC BY V4.0, Open Access
Journal article
Genomic Comparison of Translocating and Non-Translocating Escherichia coli
PLoS One, Vol.10(8), pp.1-13
2015
Abstract
Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study the genomes of four TEC strains isolated from the mesenteric lymph nodes of a fatal case of hospitalised patient (HMLN-1), blood of pigs after experimental shock (PC-1) and after non-lethal haemorrhage in rats (KIC-1 and KIC-2) were sequenced in order to identify the genes associated with their adhesion and/or translocation. To facilitate the comparison, the genomes of a non-adhering, non-translocating E. coli (46-4) and adhering but non-translocating E. coli (73-89) were also sequenced and compared. Whole genome comparison revealed that three (HMLN-1, PC-1 and KIC-2) of the four TEC strains carried a genomic island that encodes a Type 6 Secretion System that may contribute to adhesion of the bacteria to gut epithelial cells. The human TEC strain HMLN-1 also carried the invasion ibeA gene, which was absent in the animal TEC strains and is likely to be associated with host-specific translocation. Phylogenetic analysis revealed that the four TEC strains were distributed amongst three distinct E. coli phylogroups, which was supported by the presence of phylogroup specific fimbriae gene clusters. The genomic comparison has identified potential genes that can be targeted with knock-out experiments to further characterise the mechanisms of E. coli translocation.
Details
- Title
- Genomic Comparison of Translocating and Non-Translocating Escherichia coli
- Authors
- Nathan Bachmann (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAdam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- PLoS One, Vol.10(8), pp.1-13
- Publisher
- Public Library of Science
- Date published
- 2015
- DOI
- 10.1371/journal.pone.0137131
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2015 Bachmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- Data Availability
- Data Availability: All genome files are available from the National Center for Biotechnology Information (accession numbers PRJNA284559-PRJNA284564).
- Organisation Unit
- School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449355402621
- Output Type
- Journal article
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