Genome-wide scans reveal cryptic population structure in a dry-adapted eucalypt

Dorothy A Steane; Brad M Potts; Elizabeth McLean; Lesley Collins; Suzanne M Prober; William D Stock; Rene E Vaillancourt; Margaret Byrne

doi:10.1007/s11295-015-0864-z

Back

Genome-wide scans reveal cryptic population structure in a dry-adapted eucalypt

Journal article

Peer reviewed

Genome-wide scans reveal cryptic population structure in a dry-adapted eucalypt

Dorothy A Steane, Brad M Potts, Elizabeth McLean, Lesley Collins, Suzanne M Prober, William D Stock, Rene E Vaillancourt and Margaret Byrne

Tree Genetics & Genomes, Vol.11(33)

2015

DOI: https://doi.org/10.1007/s11295-015-0864-z

Files and links (1)

url

https://doi.org/10.1007/s11295-015-0864-zView

Published Version

Abstract

ecotypic variation

soil phosphorus

eucalyptus

outlier analysis

DArTseq

population genetics

Genome-wide DArTseq scans of 268 individuals of Eucalyptus salubris, distributed along an aridity gradient in southwestern Australia, revealed cryptic population structure that appears to signal hitherto unappreciated ecotypic differentiation and barriers to gene flow. Genome-wide scans were undertaken on 30 wild-sampled individuals from each of nine populations; 10 individuals per population were measured for habit and functional traits. DArTseq generated 16,122 high-quality markers, of which 56.3 % located to E. grandis chromosomes. Genetic affinities of the nine populations were only weakly correlated with geographic distances. Rather, populations appeared to form two distinct molecular lineages that maintained their distinctiveness in an area of geographic overlap. Twenty-four outlier markers signalled divergent selection and differentiation of the two putative lineages. Populations from the two lineages were phenotypically differentiated in leaf thickness, specific leaf area (SLA) and leaf nitrogen per unit mass (Nmass). The more northerly lineage (with thinner leaves) occurred in hotter, drier conditions with higher radiation. Populations of the more southerly lineage occurred on soils that were relatively low in phosphorus; the trees had thicker leaves, lower SLA and lower leaf Nmass, consistent with general responses to low nutrient levels. While historic isolation and drift may have contributed to the cryptic population structure observed, there is evidence of ecotypic adaptation, which may provide an exogenous barrier to gene flow. This study highlights the power of new molecular technologies to provide novel insights into the genetic architecture of wild populations.

Details

Title: Genome-wide scans reveal cryptic population structure in a dry-adapted eucalypt
Authors: Dorothy A Steane (Author) - University of the Sunshine Coast
Brad M Potts (Author) - University of Tasmania
Elizabeth McLean (Author) - CSIRO Land and Water Flagship
Lesley Collins (Author) - University College of Learning, New Zealand
Suzanne M Prober (Author) - CSIRO Land and Water Flagship
William D Stock (Author) - Edith Cowan University
Rene E Vaillancourt (Author) - University of Tasmania
Margaret Byrne (Author) - Western Australia Department of Parks and Wildlife
Publication details: Tree Genetics & Genomes, Vol.11(33)
Publisher: Springer
Date published: 2015
DOI: 10.1007/s11295-015-0864-z
ISSN: 1614-2942
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449820702621
Output Type: Journal article

Metrics

18 File views/ downloads

754 Record Views

30 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Forestry; Genetics & Heredity; Horticulture