Journal article
Genetic variations in E6, E7 and the long control region of human papillomavirus type 16 among patients with cervical lesions in Xinjiang, China
Cancer Cell International, Vol.19, 65
2019
Abstract
Background: Xinjiang is one of the areas with the highest incidence of cervical cancer in China. Genetic variation in Human papillomavirus type 16 (HPV16) may increase the ability of the virus to mediate carcinogenesis and immune escape, which are risk factors for the progression of cervical cancer. We investigated polymorphism in HPV16 and the distribution of its sub-lineages in the region by analyzing the E6, E7 and long control region (LCR) gene sequences from women with HPV16-positive cervical samples in Xinjiang. Methods: A total of 138 cases of cervical lesions and squamous cell carcinoma with infection of HPV16 virus were collected. The E6 and E7 genes and LCR of HPV16 virus were sequenced and compared with the HPV16 European prototype reference and other HPV16 mutants for single nucleotide polymorphisms. Neighbor-joining phylogenetic trees were constructed using E6, E7 and LCR sequences. Results: Fourteen missense mutations were found in the E6 gene; the loci with the highest mutation frequency were T350G (36/75, 48%) and T178G (19/75, 25.3%). In the E7 gene, the locus with the highest mutation frequency was A647G (18/75, 24%). A total of 33 polymorphic sites were found in the LCR, of which T7447C (39/95, 40.1%) was the most frequent. Conclusion: HPV16 in Xinjiang is mainly of the European variant, followed by the Asian variant type; no Africa 1, 2 or Asia-America variant types were found.
Details
- Title
- Genetic variations in E6, E7 and the long control region of human papillomavirus type 16 among patients with cervical lesions in Xinjiang, China
- Authors
- Xiangyi Zhe (Author) - Shihezi University, ChinaHuizhen Xin (Author) - Shihezi University, ChinaZhenzhen Pan (Author) - Xinjiang Production and Construction Corps of the Fourth Division Hospital, ChinaFuyuan Jin (Author) - Shihezi University, ChinaWeinan Zheng (Author) - Shihezi University, ChinaHongtao Li (Author) - Shihezi University, ChinaDongmei Li (Author) - Shihezi University, ChinaDongdong Coa (Author) - Shihezi University, ChinaYing Li (Author) - Shihezi University, ChinaChunhe Zhang (Author) - Shihezi University, ChinaShaowei Fu (Author) - Shihezi University, ChinaRenfu Shao (Author) - University of the Sunshine Coast - School of Science & EngineeringZemin Pan (Author) - Shihezi University, China
- Publication details
- Cancer Cell International, Vol.19, 65; 11
- Publisher
- BioMed Central Ltd.
- Date published
- 2019
- DOI
- 10.1186/s12935-019-0774-5
- ISSN
- 1475-2867
- Copyright note
- Copyright © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451000102621
- Output Type
- Journal article
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