Genetic diversity and virulence characteristics of biofilm-producing uropathogenic Escherichia coli

Ali Qasemi; Fateh Rahimi; Mohammad Katouli

doi:10.1007/s10123-021-00221-w

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Genetic diversity and virulence characteristics of biofilm-producing uropathogenic Escherichia coli

Journal article

Peer reviewed

Genetic diversity and virulence characteristics of biofilm-producing uropathogenic Escherichia coli

Ali Qasemi, Fateh Rahimi and Mohammad Katouli

International Microbiology, Vol.25, pp.297-307

2021

DOI: https://doi.org/10.1007/s10123-021-00221-w

PMID: 34705131

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Abstract

UTI

UPEC

biofilm

virulence factors

phylogenetic groups

Uropathogenic E. coli (UPEC) strains exhibit different levels of biofilm formation that help adhesion of the bacteria to uroepithelial cells. We investigated the genetic diversity and virulence-associated genes (VAGs) of biofilm-producing UPEC. A collection of 107 biofilm-producing (BFP) UPEC strains isolated from patients with UTI in Iran were divided into three groups of strong, moderate, and weak BFPs after a quantitative microtiter plate assay, and the involvement of curli and cellulose in adhesion of the strains to T24 cell line was confirmed by the construction of csgD and yedQ mutants of two representative UPEC strains. BFP strains were tested for their genetic diversity, phylogenetic groups, and the presence of 15 VAGs. A significant decrease in adhesion of csgD and yedQ mutant strains confirmed the role of biofilm production in adhesion to uroepithelial cells. A high diversity was found among all three groups of strong (Di = 0.998), moderate (Di = 0.998), and weak (Di = 0.988) BFPs with majority of the strains belonging to phylogroups B2 (44.9%) and A (24.3%). Strong BFP strains carried significantly higher level papEF, hlyA, and iutA than other BFP groups. In contrast, the presence of fimH, focG, sfaS, set-1, and cvaC was more pronounced among weak BFP strains. There exists a high genetic diversity among the BFP strains with different VGA profiles. However, the high prevalence of phylogroup A among BFP strains suggests the fitness of commensal E. coli strains to cause UTI in this country.

Details

Title: Genetic diversity and virulence characteristics of biofilm-producing uropathogenic Escherichia coli
Authors: Ali Qasemi (Author) - University of Isfahan
Fateh Rahimi (Corresponding Author) - University of Isfahan
Mohammad Katouli (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Publication details: International Microbiology, Vol.25, pp.297-307
Publisher: Springer Nature Switzerland AG
Date published: 2021
DOI: 10.1007/s10123-021-00221-w
ISSN: 1618-1905
PMID: 34705131
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99578905402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology

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