Journal article
Genes and associated peptides involved with aestivation in a land snail
General and Comparative Endocrinology, Vol.246, pp.88-98
2017
Abstract
Some animals can undergo a remarkable transition from active normal life to a dormant state called aestivation; entry into this hypometabolic state ensures that life continues even during long periods of environmental hardship. In this study, we aimed to identify those central nervous system (CNS) peptides that may regulate metabolic suppression leading to aestivation in land snails. Mass spectral-based neuropeptidome analysis of the CNS comparing active and aestivating states, revealed 19 differentially produced peptides; 2 were upregulated in active animals and 17 were upregulated in aestivated animals. Of those, the buccalin neuropeptide was further investigated since there is existing evidence in molluscs that buccalin modulates physiology by muscle contraction. The T. pisana CNS contains two buccalin transcripts that encode precursor proteins that are capable of releasing numerous buccalin peptides. Of these, Tpi-buccalin-2 is most highly expressed within our CNS transcriptome derived from multiple metabolic states. No significant difference was observed at the level of gene expression levels for Tpi-buccalin-2 between active and aestivated animals, suggesting that regulation may reside at the level of post-translational control of peptide abundance. Spatial gene and peptide expression analysis of aestivated snail CNS demonstrated that buccalin-2 has widespread distribution within regions that control several physiological roles. In conclusion, we provide the first detailed molecular analysis of the peptides and associated genes that are related to hypometabolism in a gastropod snail known to undergo extended periods of aestivation.
Details
- Title
- Genes and associated peptides involved with aestivation in a land snail
- Authors
- Kevin J Adamson (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringBronwyn Rotgans (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringThanapong Kruangkum (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAnna V Kuballa (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringK B Storey (Author) - Carleton University, CanadaScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- General and Comparative Endocrinology, Vol.246, pp.88-98
- Publisher
- Academic Press
- Date published
- 2017
- DOI
- 10.1016/j.ygcen.2015.10.013
- ISSN
- 0016-6480
- Copyright note
- Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Health - Biomedicine; Faculty of Science, Health, Education and Engineering; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449168102621
- Output Type
- Journal article
Metrics
162 File views/ downloads
1209 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Endocrinology & Metabolism
- Zoology