Files and links (1)
Published VersionCC BY V4.0, Open Access
Journal article
Gene methylation of CADM1 and MAL identified as a biomarker of high grade anal intraepithelial neoplasia
Scientific Reports, Vol.12(1), pp.1-11
2022
PMID: 35241698
Abstract
Human papillomavirus (HPV) is detected in up to 96% of anal squamous cell cancers, where screening programs needed. However, the best methodology is still undetermined. Host DNA methylation markers CADM1, MAL and miR124 have been identified in cervical disease, but not anal disease. Anal swabs varying by disease grade were assessed for DNA methylation of CADM1, MAL and miR124-2. Each marker was compared across disease grades, stratified by HPV and HIV status. Receiver operating characteristic curves identified the predictive value of significant gene candidates. CADM1 methylation was significantly higher in high-grade squamous intraepithelial lesions (HSIL) compared with low-grade (LSIL) (p = 0.005) or normal (p < 0.001) samples with 67.2% correctly identified as HSIL. MAL methylation was significantly (p = 0.002) increased in HSIL compared with LSIL in HIV positive participants with 79.8% correctly indicated as HSIL. Gene miR124-2, showed no difference between disease grades. Biomarkers with established diagnostic value in cervical disease have limited utility in the prediction of anal disease, with CADM1 identified as a marker with screening potential in a gay and bisexual men (GBM) population and MAL in HIV positive GBM population. New markers specific to the anal mucosa are required to improve triage of high-risk individuals.
Details
- Title
- Gene methylation of CADM1 and MAL identified as a biomarker of high grade anal intraepithelial neoplasia
- Authors
- Samuel Phillips (Corresponding Author) - The University of MelbourneKahli Cassells - The University of MelbourneSuzanne M Garland - Royal Women's HospitalDorothy A Machalek - UNSW SydneyJennifer M Roberts - Douglass Hanly Moir PathologyDavid J Templeton - Sydney Local Health DistrictFengyi Jin - UNSW SydneyI Mary Poynten - UNSW SydneyRichard J Hillman - St Vincent's Hospital MelbourneAndrew E Grulich - UNSW SydneyGerald Murray - The University of MelbourneSepehr N Tabrizi - The University of MelbourneMonica Molano - Royal Women's HospitalAlyssa M Cornall - The University of MelbourneSPANC Team (Research Group)
- Publication details
- Scientific Reports, Vol.12(1), pp.1-11
- Publisher
- Nature Publishing Group
- Date published
- 2022
- DOI
- 10.1038/s41598-022-07258-5
- ISSN
- 2045-2322
- PMID
- 35241698
- Copyright note
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
- Data Availability
- All data and materials are available within this document and the supplementary files.
- Grant note
- This work was supported by National Health and Medical Research Council Program Grant (Sexually Transmitted Infections: causes, consequences, and interventions; grant no. 568971; to A.E. Grulich), Cancer Council New South Wales Strategic Research Partnership Program Grant (Preventing morbidity and mortality from anal cancer; Grant No. 13-11; to A.E. Grulich, I.M. Poynten, F. Jin, RJ Hillman, DJ Templeton, JM Roberts), and Cancer Council Victoria grant for evaluating molecular biomarkers of anal cancer risk (application no. APP1130507; to S.M. Garland, M. Molano, A.M. Cornall).
- Organisation Unit
- School of Health - Biomedicine; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991144231002621
- Output Type
- Journal article
Metrics
7 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Oncology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites