Gene identification and evidence for expression of G protein alpha subunits, phospholipase C, and an inositol 1,4,5-trisphosphate receptor in Aplysia californica rhinophore

Scott F Cummins; Melissa R De Vries; K S Hill; D Boehning; G T Nagle

doi:10.1016/j.ygeno.2007.03.020

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Gene identification and evidence for expression of G protein alpha subunits, phospholipase C, and an inositol 1,4,5-trisphosphate receptor in Aplysia californica rhinophore

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Gene identification and evidence for expression of G protein alpha subunits, phospholipase C, and an inositol 1,4,5-trisphosphate receptor in Aplysia californica rhinophore

Scott F Cummins, Melissa R De Vries, K S Hill, D Boehning and G T Nagle

Genomics, Vol.90(1), pp.110-120

2007

DOI: https://doi.org/10.1016/j.ygeno.2007.03.020

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Abstract

Fisheries Sciences

Aplysia

pheromonal communication

signal transduction

In the marine mollusk Aplysia californica, waterborne protein pheromones that are released during egg laying act in concert to stimulate mate attraction. However, molecular information concerning the cellular receptors and signaling mechanisms that may be involved in waterborne peptide and protein pheromonal communication is lacking. As a first step toward examining whether members of the G protein family and phosphoinositide signaling pathway are present in the primary peripheral chemosensory organs (i.e., rhinophores), we isolated five full-length cDNA clones from an A. californica central nervous system cDNA library. These clones encoded (1) the G protein alpha subunits of the Gq, Gi, and Go families, (2) a protein with homology to phospholipase C (PLC) isoforms, and (3) an inositol 1,4,5-trisphosphate receptor (IP3R). The expression of these genes was examined using laser capture microdissection/reverse transcription-polymerase chain reaction and in situ hybridization. All of them are expressed in the rhinophore sensory epithelium, suggesting that Galphaq, Galphai, Galphao, PLC-like protein, and IP3R may be involved in waterborne protein pheromone detection in Aplysia-possibly via a phosphoinositide signaling mechanism.

Details

Title: Gene identification and evidence for expression of G protein alpha subunits, phospholipase C, and an inositol 1,4,5-trisphosphate receptor in Aplysia californica rhinophore
Authors: Scott F Cummins (Author) - University of Texas, United States
Melissa R De Vries (Author) - University of Texas, United States
K S Hill (Author) - University of Texas, United States
D Boehning (Author) - University of Texas, United States
G T Nagle (Author) - University of Texas, United States
Publication details: Genomics, Vol.90(1), pp.110-120
Publisher: Academic Press
Date published: 2007
DOI: 10.1016/j.ygeno.2007.03.020
ISSN: 0888-7543
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449872602621
Output Type: Journal article

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