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Gene expression in response to exercise in patients with chronic fatigue syndrome: A pilot study
Journal article   Open access   Peer reviewed

Gene expression in response to exercise in patients with chronic fatigue syndrome: A pilot study

Andrew Keech, Ute Vollmer-Conna, Benjamin K. Barry and Andrew R. Lloyd
Frontiers in Physiology, Vol.7, pp.1-7
2016
PMID: 27713703
pdf
fphys-07-00421877.09 kBDownloadView
Published Version Open Access CC BY V4.0
url
https://doi.org/10.3389/fphys.2016.00421View
Published Version Open CC BY V4.0

Abstract

Central sensitisation MRNA Myalgic encephalomyelitis Pathogenesis Post-exertional malaise
Chronic fatigue syndrome (CFS) is a debilitating disorder of unknown pathogenesis, characterized by fatigue, which is exacerbated after minimal exercise. We examined the effect of a single bout of aerobic exercise on leucocyte mRNA expression of genes putatively linked to exaggerated afferent signaling as an under-pinning of the fatigue state. A carefully-characterized sample of patients with CFS (N = 10) and healthy matched control participants (N = 12) were included. Participant ratings of fatigue and other symptoms, as well as blood samples, were obtained at baseline, and five other time-points up to 72 h after 25 min of moderate-intensity cycling exercise. Leucocyte mRNA of 19 metabolite-sensing, adrenergic, immune, and neurotransmission genes was examined using quantitative polymerase chain reaction. Patients with CFS reported substantial fatigue, functional impairment, and poor sleep at baseline (all p < 0.02), and exercise immediately induced worsened patients' fatigue (effect size, ES = 1.17). There were no significant changes in gene expression after exercise and patients did not differ from control participants at any time point. Higher levels of expression of ficolin (FCN1) and a purinergic receptor (P2RX4) in patients with CFS were found when all time points were combined. Patients with CFS did not show significant exercise-induced changes in leucocyte mRNA of 19 metabolite-sensing, adrenergic, immune and neurotransmission genes despite a prominent exacerbation of fatigue.

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