Gene Expression for Carbonic Anhydrase Isoenzymes in Human Nasal Mucosa

A S Tarum; B Bryant; W Zhai; Colin Solomon; D Shusterman

doi:10.1093/chemse/bjg054

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Gene Expression for Carbonic Anhydrase Isoenzymes in Human Nasal Mucosa

Journal article

Peer reviewed

Gene Expression for Carbonic Anhydrase Isoenzymes in Human Nasal Mucosa

A S Tarum, B Bryant, W Zhai, Colin Solomon and D Shusterman

Chemical Senses, Vol.28(7), pp.621-629

2003

DOI: https://doi.org/10.1093/chemse/bjg054

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Abstract

Biological Sciences

carbon dioxide

carbonic anhydrase

gene expression

nasal epithelium

nasal irritation

quantitative PCR

Carbonic anhydrase (CA) is physiologically important in the reversible hydration reaction of CO2; it is expressed in a number of isoforms (CA I-XIV) with varying degrees of enzymatic activity. In nasal chemesthesis, CA inhibition decreases the electrophysiologic response to CO2, a common irritant test compound. CA enzymatic activity has been demonstrated in the human nasal mucosa using enzyme histochemical methods, but no systematic study of nasal mucosal CA isoenzyme gene expression has been published. We examined CA gene expression in superficial nasal mucosal scrapings from 15 subjects (6 females; 6 allergic rhinitics; age range, 21-56 years). Both non-quantitative and quantitative reverse transcription polymerase chain reaction (RT-PCR) were performed using primers for each gene coding for the 11 catalytically active CA isoenzymes and the housekeeping gene GADPH. Amplification products of GADPH and 10 of the 11 CA genes were detected in the specimens (CA VA was not detected). Relative expression of the CA genes was quantified using real-time PCR. Averaged across subjects, the relative abundance of the CA isoenzyme transcripts is as follows: CA XII > CA II > CA VB > CA IV > CA IX > CA III > CA XIV > CA I > CA VI > CA VII. Limited qualitative validation of gene expression was obtained by immunohistochemistry for CA I, CA II and CA IV. We also observed inter-individual variability in the expression of CA isoenzymes in human nasal mucosa, potentially contributing to differences in nasal chemosensitivity to CO2 between individuals.

Details

Title: Gene Expression for Carbonic Anhydrase Isoenzymes in Human Nasal Mucosa
Authors: A S Tarum (Author) - University of California, United States
B Bryant (Author) - Moell Chemical Senses Center, United States
W Zhai (Author) - University of California, United States
Colin Solomon (Author) - University of California, United States
D Shusterman (Author) - University of California, United States
Publication details: Chemical Senses, Vol.28(7), pp.621-629
Publisher: Oxford University Press
Date published: 2003
DOI: 10.1093/chemse/bjg054
ISSN: 0379-864X
Organisation Unit: Centre for Human Factors and Systems Science; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450305302621
Output Type: Journal article

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