Journal article
GPCR and IR genes in Schistosoma mansoni miracidia
Parasites & vectors, Vol.9, pp.1-12
2016
PMCID: PMC5080760
PMID: 27784323
Abstract
Background: Schistosoma species are responsible for the disease schistosomiasis, a highly prevalent helminthic disease that requires a freshwater snail as intermediate host. The S. mansoni free-living miracidium must utilize olfaction to find a suitable snail host, and certain types of rhodopsin G protein-coupled receptors (GPCRs) and ionotropic receptors (IRs) have been identified as olfactory receptors in other animal phyla. The Schistosoma genome project, together with the recent availability of proteomic databases, allowed for studies to explore receptors within S. mansoni, some of which may contribute to host finding. Results: We have identified 17 rhodopsin-type GPCR sequences in S. mansoni belonging to four subclasses, including ligand-specific GPCRs (i.e. neuropeptide and opsin). RT-PCR demonstrated the expression of nine out of the 17 GPCRs in the free-living miracidia, each of which have been characterized for homology to S. haematobium. Among the nine GPCRs, two are predicted as Gq-opsins. We also describe the characterization of a Schistosoma-encoded IR based on similarity with other species IR and conservation of IR-like domains. Schistosoma mansoni IR is expressed in miracidia at 3 and 6 h post-hatch. Conclusions: The identification of receptors in S. mansoni miracidia, presented here, contributes not only to further understanding of Schistosoma biology and signal transduction but also provides a basis for approaches that may modify parasite behaviour.
Details
- Title
- GPCR and IR genes in Schistosoma mansoni miracidia
- Authors
- Di Liang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMin Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringDonald P McManus (Author) - QIMR Berghofer Medical Research InstituteScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Parasites & vectors, Vol.9, pp.1-12
- Publisher
- BioMed Central Ltd.
- Date published
- 2016
- DOI
- 10.1186/s13071-016-1837-2
- ISSN
- 1756-3305
- PMID
- 27784323; PMC5080760
- Copyright note
- Copyright © The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Data Availability
- The original S. mansoni protein dataset is available from the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases.
- Grant note
- We thank the University of the Sunshine Coast who provided an internal grant to help support this work (TW).
- Organisation Unit
- School of Science and Engineering - Legacy; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451055602621
- Output Type
- Journal article
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