Journal article
Fragmentations of [M − H]− anions of peptides containing tyrosine sulfate. Does the sulfate group rearrange? A joint experimental and theoretical study
Rapid Communications in Mass Spectrometry, Vol.27(10), pp.1135-1142
2013
Abstract
RATIONALE: To investigate the fragmentations in the negative-ion electrospray mass spectra of peptides containing tyrosine sulfate. METHODS: Possible fragmentation mechanisms were explored using a Waters QTOF2 tandem mass spectrometer in concert with calculations at the CAM-B3LYP/6-311++g(d,p) level of theory. RESULTS: The major negative ion formed in the ESI-MS of peptides containing tyrosine sulfate is [(M - H) - SO3]- and this process normally yields the base peak of the spectrum. The basic backbone cleavages of [(M - H) - SO3]- allowed the sequence of the peptide to be determined. Rearrangement reactions involving the formation of HOSO3- and [(M - H)-H2SO4]- yielded minor peaks with relative abundances ≤ 10% and ≤ 2%, respectively. CONCLUSIONS: The mass spectra of the [M - H]- and [(M - H)-SO3]- anions of peptides containing tyrosine sulfate allowed the position of the tyrosine sulfate group to be determined, together with the amino acid sequence of the peptide.
Details
- Title
- Fragmentations of [M − H]− anions of peptides containing tyrosine sulfate. Does the sulfate group rearrange? A joint experimental and theoretical study
- Authors
- T T Nha Tran (Author) - University of AdelaideTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringS Hack (Author) - University of AdelaideJ H Bowie (Author) - University of Adelaide
- Publication details
- Rapid Communications in Mass Spectrometry, Vol.27(10), pp.1135-1142
- Publisher
- John Wiley & Sons Ltd.
- Date published
- 2013
- DOI
- 10.1002/rcm.6547
- ISSN
- 0951-4198; 0951-4198
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448996602621
- Output Type
- Journal article
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