Journal article
Formulation of DNA chimera templates: Effects on emission behavior of silver nanoclusters and sensing
Analytica Chimica Acta, Vol.1010, pp.62-68
2018
Abstract
Single strand DNA (ssDNA) chimeras consisting of a silver nanoclusters-nucleating sequence (NC) and an aptamer are widely employed to synthesize functional silver nanoclusters (AgNCs) for sensing purpose. Despite its simplicity, this chimeric-templated AgNCs often leads to undesirable turn-off effect, which may suffer from false positive signals caused by interference. In our effort to elucidate how the relative position of NC and aptamer affects the fluorescence behavior and sensing performance, we systematically formulated these NC and aptamer regions at different position in a DNA chimera. Using adenosine aptamer as a model, we tested the adenosine-induced optical response of each design. We also investigated the effect of linker region connecting NC and aptamer, as well as different NC sequence on the sensing performance. We concluded that locating NC sequence at 5'-end exhibited the best response, with immediate fluorescence enhancement observed over a wide linear range (1-2500 mu M). Our experimental findings help to explain the emission behavior and sensing performance of chimeric conjugates of AgNCs, providing an important means to formulate a better aptasensor. (c) 2018 Elsevier B.V. All rights reserved.
Details
- Title
- Formulation of DNA chimera templates: Effects on emission behavior of silver nanoclusters and sensing
- Authors
- Shi Ting Lee (Author) - University of Nottingham Malaysia, MalaysiaDavid Beaumont (Author) - University of Nottingham, United KingdomXuai Du Su (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringKasturi Muthoosamy (Author) - University of Nottingham Malaysia, MalaysiaSiu Yee New (Author) - University of Nottingham Malaysia, Malaysia
- Publication details
- Analytica Chimica Acta, Vol.1010, pp.62-68
- Publisher
- Elsevier BV
- Date published
- 2018
- DOI
- 10.1016/j.aca.2018.01.012
- ISSN
- 0003-2670
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99451240702621
- Output Type
- Journal article
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