Flash chronopotentiometric sensing of the polyions protamine and heparin at ion-selective membranes

K L Gemene; Eric Bakker

doi:10.1016/j.ab.2008.12.023

Back

Flash chronopotentiometric sensing of the polyions protamine and heparin at ion-selective membranes

Journal article

Peer reviewed

Flash chronopotentiometric sensing of the polyions protamine and heparin at ion-selective membranes

K L Gemene and Eric Bakker

Analytical Biochemistry, Vol.386(2), pp.276-281

2009

DOI: https://doi.org/10.1016/j.ab.2008.12.023

Files and links (1)

url

https://doi.org/10.1016/j.ab.2008.12.023View

Published Version

Abstract

protamine

heparin

anticoagulant

blood analysis

clinical diagnostics

We report here on a highly sensitive and rapid detection technique, multipulse flash chronopotentiometry, for the anticoagulant polyion heparin and its antidote protamine. The technique is based on a localized titration of the polyions at the surface of an appropriately formulated polymeric ion-selective membrane devoid of ion exchange properties to prohibit spontaneous extraction processes. A defined ion flux from the sample side to the membrane is induced electrochemically by applying a current pulse of appropriate amplitude and sign. The resulting depletion of the measured ions at the membrane surface gives rise to a characteristic limiting current or transition time and is observed as an inflection point in the resulting chronopotentiogram. The limiting current and the square root of the transition time are linear functions of the concentration of the polyion and yield sensitive and rapid analytical information attractive for clinical diagnostics applications. The polyion protamine is detected in 10-fold diluted blood samples in a matter of seconds via a cathodic current pulse. The utility of the technique for monitoring heparin/protamine titrations in physiological saline solutions is demonstrated.

Details

Title: Flash chronopotentiometric sensing of the polyions protamine and heparin at ion-selective membranes
Authors: K L Gemene (Author) - Purdue University, United States
Eric Bakker (Author) - Curtin University
Publication details: Analytical Biochemistry, Vol.386(2), pp.276-281
Publisher: Elsevier Inc.
Date published: 2009
DOI: 10.1016/j.ab.2008.12.023
ISSN: 0003-2697
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450395402621
Output Type: Journal article

Metrics

4 File views/ downloads

323 Record Views

22 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical