Journal article
Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters
Canadian Journal of Microbiology, Vol.66(11), pp.623-630
2020
Abstract
We investigated the specificity and sensitivity of two horse-associated markers, HoF597 and Horse mtCytb, and 12 mitochondrial and bacterial markers of six animal species (human, cow, pig, bird, dog, chicken) in the faecal samples of 50 individual horses. Both horse markers were detected in 48 (96%) faecal samples. Cross-reactivity with dog (BacCan545) and pig (P23-2) occurred in 88% and 72% of horse faecal samples, respectively. Several other bacterial and mitochondrial markers of non-target hosts were also detected; however, their specificities were >80%. Analyses of samples from surface waters (n = 11) on or adjacent to properties from which horse faecal samples had been collected showed only the presence of HoF597 but not horse mitochondrial marker. Our data suggest that while bacterial and (or) mitochondrial markers of other animal species may be present in horse faeces, dog and pig markers may predominantly be present in horse faecal samples, which points to their nonspecificity as markers for microbial source tracking. Although HoF597 and Horse mtCytb are highly sensitive and specific for the detection of horse faecal pollution, because of their low numbers, mitochondrial (mtDNA) markers may not be robust for screening surface waters.
Details
- Title
- Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters
- Authors
- Jessica Gray (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyNicole Masters (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyAaron Wiegand (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyMohammad Katouli (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
- Publication details
- Canadian Journal of Microbiology, Vol.66(11), pp.623-630
- Publisher
- Canadian Science Publishing
- DOI
- 10.1139/cjm-2019-0499
- ISSN
- 1480-3275
- Organisation Unit
- Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; School of Health
- Language
- English
- Record Identifier
- 99467305302621
- Output Type
- Journal article
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- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Immunology
- Microbiology
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Source: InCites