Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters

Jessica Gray; Nicole Masters; Aaron Wiegand; Mohammad Katouli

doi:10.1139/cjm-2019-0499

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Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters

Journal article

Peer reviewed

Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters

Jessica Gray, Nicole Masters, Aaron Wiegand and Mohammad Katouli

Canadian Journal of Microbiology, Vol.66(11), pp.623-630

2020

DOI: https://doi.org/10.1139/cjm-2019-0499

PMID: 32692953

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Abstract

field assessment

HoF597 marker

microbial source tracking

mtCytb

surface waters

We investigated the specificity and sensitivity of two horse-associated markers, HoF597 and Horse mtCytb, and 12 mitochondrial and bacterial markers of six animal species (human, cow, pig, bird, dog, chicken) in the faecal samples of 50 individual horses. Both horse markers were detected in 48 (96%) faecal samples. Cross-reactivity with dog (BacCan545) and pig (P23-2) occurred in 88% and 72% of horse faecal samples, respectively. Several other bacterial and mitochondrial markers of non-target hosts were also detected; however, their specificities were >80%. Analyses of samples from surface waters (n = 11) on or adjacent to properties from which horse faecal samples had been collected showed only the presence of HoF597 but not horse mitochondrial marker. Our data suggest that while bacterial and (or) mitochondrial markers of other animal species may be present in horse faeces, dog and pig markers may predominantly be present in horse faecal samples, which points to their nonspecificity as markers for microbial source tracking. Although HoF597 and Horse mtCytb are highly sensitive and specific for the detection of horse faecal pollution, because of their low numbers, mitochondrial (mtDNA) markers may not be robust for screening surface waters.

Details

Title: Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters
Authors: Jessica Gray (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Nicole Masters (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Aaron Wiegand (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Mohammad Katouli (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Publication details: Canadian Journal of Microbiology, Vol.66(11), pp.623-630
Publisher: Canadian Science Publishing
Date published: 2020
DOI: 10.1139/cjm-2019-0499
ISSN: 1480-3275
PMID: 32692953
Organisation Unit: School of Science and Engineering - Legacy; School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99467305302621
Output Type: Journal article

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