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Br J Haematol - 2023 - McGowan - Feasibility for non‐invasive prenatal fetal blood group and platelet genotyping by1.23 MB
Published VersionCC BY-NC V4.0, Open Access
Journal article
Feasibility for non-invasive prenatal fetal blood group and platelet genotyping by massively parallel sequencing: A single test system for multiple atypical red cell, platelet and quality control markers
British Journal of Haematology, Vol.204(2), pp.694-705
2024
Abstract
Non-invasive prenatal tests (NIPT) to predict fetal red cell or platelet antigen status for alloimmunised women are provided for select antigens. This study reports on massively parallel sequencing (MPS) using a red cell and platelet probe panel targeting multiple nucleotide variants, plus individual identification single nucleotide polymorphisms (IISNPs). Maternal blood samples were provided from 33 alloimmunised cases, including seven with two red cell antibodies. Cell-free and genomic DNA was sequenced using targeted MPS and bioinformatically analysed using low-frequency variant detection. The resulting maternal genomic DNA allele frequency was subtracted from the cell-free DNA counterpart. Outcomes were matched against validated phenotyping/genotyping methods, where available. A 2.5% subtractive allele frequency threshold was set after comparing MPS predictions for K, RhC/c, RhE/e and Fya /Fyb against expected outcomes. This threshold was used for subsequent predictions, including HPA-15a, Jka /Jkb , Kpa /Kpb and Lua . MPS outcomes were 97.2% concordant with validated methods; one RhC case was discordantly negative and lacked IISNPs. IISNPs were informative for 30/33 cases as controls. NIPT MPS is feasible for fetal blood group genotyping and covers multiple blood groups and control targets in a single test. Noting caution for the Rh system, this has the potential to provide a personalised service for alloimmunised women.
Details
- Title
- Feasibility for non-invasive prenatal fetal blood group and platelet genotyping by massively parallel sequencing: A single test system for multiple atypical red cell, platelet and quality control markers
- Authors
- Eunike C McGowan (Author) - Australian Red Cross LifebloodHelen O'Brien (Author) - Australian Red Cross LifebloodMia E Sarri (Author) - Australian Red Cross LifebloodGenghis H Lopez (Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - LegacyJames J Daly (Author) - Australian Red Cross LifebloodRobert L Flower (Author) - Australian Red Cross LifebloodGlenn J Gardener (Author) - Mater Mothers' HospitalCatherine A Hyland (Corresponding Author) - Australian Red Cross Lifeblood
- Publication details
- British Journal of Haematology, Vol.204(2), pp.694-705
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2024
- DOI
- 10.1111/bjh.19197
- ISSN
- 1365-2141; 0007-1048
- Copyright note
- © 2023 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99982895902621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Hematology
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